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dc.contributor.authorSales, Naiara Guimaraes
dc.contributor.authorWangensteen Fuentes, Owen Simon
dc.contributor.authorCarvalho, Daniel C.
dc.contributor.authorDeiner, Kristy
dc.contributor.authorPræbel, Kim
dc.contributor.authorCoscia, Ilaria
dc.contributor.authorMcDevitt, Allan D
dc.contributor.authorMariani, Stefano
dc.date.accessioned2020-10-01T09:25:25Z
dc.date.available2020-10-01T09:25:25Z
dc.date.issued2021-09-01
dc.description.abstractThe biodiverse Neotropical ecoregion remains insufficiently assessed, poorly managed, and threatened by unregulated human activities. Novel, rapid and cost-effective DNA-based approaches are valuable to improve understanding of the biological communities and for biomonitoring in remote areas. Here, we evaluate the potential of environmental DNA (eDNA) metabarcoding for assessing the structure and distribution of fish communities by analysing water and sediment from 11 locations along the Jequitinhonha River catchment (Brazil). Each site was sampled twice, before and after a major rain event in a five-week period and fish diversity was estimated using high-throughput sequencing of 12S rRNA amplicons. In total, 252 Molecular Operational Taxonomic Units (MOTUs) and 34 fish species were recovered, including endemic, introduced, and previously unrecorded species for this basin. Spatio-temporal variation of eDNA from fish assemblages was observed and species richness was nearly twice as high before the major rain event compared to afterwards. Yet, peaks of diversity were primarily associated with only four of the locations. No correlation between β-diversity and longitudinal distance or presence of dams was detected, but low species richness observed at sites located near dams might that these anthropogenic barriers may have an impact on local fish diversity. Unexpectedly high α-diversity levels recorded at the river mouth suggest that these sections should be further evaluated as putative “eDNA reservoirs” for rapid monitoring. By uncovering spatio-temporal changes, unrecorded biodiversity components, and putative anthropogenic impacts on fish assemblages, we further strengthen the potential of eDNA metabarcoding as a biomonitoring tool, especially in regions often neglected or difficult to access.en_US
dc.descriptionAccepted manuscript version, licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0. </a>en_US
dc.identifier.citationSales NG, Wangensteen Fuentes OS, Carvalho DC, Deiner K, Præbel K, Coscia I, McDevitt AD, Mariani S. Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding. Science of the Total Environment. 2021;754en_US
dc.identifier.cristinIDFRIDAID 1827590
dc.identifier.doi10.1016/j.scitotenv.2020.142096
dc.identifier.issn0048-9697
dc.identifier.issn1879-1026
dc.identifier.urihttps://hdl.handle.net/10037/19525
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalScience of the Total Environment
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© 2020 Elsevier B.V. All rights reserved.en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.titleSpace-time dynamics in monitoring neotropical fish communities using eDNA metabarcodingen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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