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dc.contributor.advisorSveberg Dietrichs, Erik
dc.contributor.advisorHuber, Sandra
dc.contributor.advisorFuskevåg, Ole-Martin
dc.contributor.advisorWestrheim Ravna, Aina
dc.contributor.authorVoloshyna, Tetyana
dc.date.accessioned2021-06-21T07:46:44Z
dc.date.available2021-06-21T07:46:44Z
dc.date.issued2021-05-18en
dc.description.abstractPerfluoroalkyl substances (PFAS) have received a great deal of attention due to the ubiquitous occurrence and persistence in the environment, resistance to degradation, and biological accumulation in wildlife and humans. PFAS are being detected around the globe, raising concerns regarding the toxicity and health risks to humans. Studies have shown that environmental toxicants, including PFAS, can interact with transporter proteins involved in the absorption and distribution, as well as detoxification, of drugs, xenobiotics and other toxicants. In this study, the effects of PFAS of different carbon chain lengths, perfluorooctane sulfonic acid (PFOS), perfluorohexane sulfonic acid (PFHxS) and perfluorobutane sulfonic acid (PFBS), on the activity of P-glycoprotein (P-gp) were investigated using the Caco-2 cell line model. Additionally, it was investigated whether PFOS, PFHxS and PFBS cross the Caco-2 cell monolayer. Two known P-gp substrates, quinidine and verapamil, were used to inhibit P-gp in Caco-2 cells, to examine whether PFOS, PFHxS and PFBS might be substrates of P-gp. No interaction between the tested compounds and digoxin were observed, suggesting that these individual PFAS did not modulate the activity of P-gp. Nevertheless, it was observed that PFOS, PFHxS and PFBS crossed the Caco-2 monolayer. Inhibition of P-gp by known inhibitors caused a slight decrease in the rate of PFOS and PFHxS, but not PFBS, in the apical to basolateral direction and in the basolateral to apical direction, suggesting that active uptake and efflux might be involved in the transport of PFOS, PFHxS, but not PFBS, across the Caco-2 monolayer.en_US
dc.identifier.urihttps://hdl.handle.net/10037/21486
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universitetno
dc.publisherUiT The Arctic University of Norwayen
dc.rights.holderCopyright 2021 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en_US
dc.subject.courseIDMBI-3911
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473en_US
dc.subjectP-glycoproteinen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471en_US
dc.subjectCaco-2en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471en_US
dc.subjectPFASen_US
dc.titleA study on the interactions between perfluorosulfonic acids and P-glycoproteinen_US
dc.typeMastergradsoppgaveno
dc.typeMaster thesisen


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Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)