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dc.contributor.authorCauzzo, Jennifer
dc.contributor.authorJayakumar, Nikhil
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorAhmad, Azeem
dc.contributor.authorSkalko-Basnet, Natasa
dc.date.accessioned2021-06-28T08:50:52Z
dc.date.available2021-06-28T08:50:52Z
dc.date.issued2021-04-21
dc.description.abstractThe rapid development of nanomedicine and drug delivery systems calls for new and effective characterization techniques that can accurately characterize both the properties and the behavior of nanosystems. Standard methods such as dynamic light scattering (DLS) and fluorescent-based assays present challenges in terms of system’s instability, machine sensitivity, and loss of tracking ability, among others. In this study, we explore some of the downsides of batch-mode analyses and fluorescent labeling, while introducing quantitative phase microscopy (QPM) as a label-free complimentary characterization technique. Liposomes were used as a model nanocarrier for their therapeutic relevance and structural versatility. A successful immobilization of liposomes in a non-dried setup allowed for static imaging conditions in an off-axis phase microscope. Image reconstruction was then performed with a phase-shifting algorithm providing high spatial resolution. Our results show the potential of QPM to localize subdiffraction-limited liposomes, estimate their size, and track their integrity over time. Moreover, QPM full-field-of-view images enable the estimation of a single-particle-based size distribution, providing an alternative to the batch mode approach. QPM thus overcomes some of the drawbacks of the conventional methods, serving as a relevant complimentary technique in the characterization of nanosystems.en_US
dc.identifier.citationCauzzo, Jayakumar, Ahluwalia, Ahmad, Skalko-Basnet. Characterization of Liposomes Using Quantitative Phase Microscopy (QPM). Pharmaceutics. 2021en_US
dc.identifier.cristinIDFRIDAID 1906341
dc.identifier.doi10.3390/pharmaceutics13050590
dc.identifier.issn1999-4923
dc.identifier.urihttps://hdl.handle.net/10037/21570
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.ispartofCauzzo, J. (2022). Microscopy Meets Nanomedicine: The Challenge of Liposomes. Selecting, Understanding, and Adapting Imaging Techniques to Localize and Characterize Nanocarriers. (Doctoral thesis). <a href=https://hdl.handle.net/10037/25291>https://hdl.handle.net/10037/25291</a>.
dc.relation.journalPharmaceutics
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/766181/EU/Super-resolution optical microscopy of nanosized pore dynamics in endothelial cells/DeLIVER/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/288565/Norway/Integrated photonic chip-based nanoscopy for pathology & the clinic//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.titleCharacterization of Liposomes Using Quantitative Phase Microscopy (QPM)en_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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