Vis enkel innførsel

dc.contributor.authorAndersen, Maria Karoline
dc.contributor.authorHøiem, Therese Stork
dc.contributor.authorClaes, Britt S.R.
dc.contributor.authorBalluff, Benjamin
dc.contributor.authorMarta, Martin-Lorenzo
dc.contributor.authorRichardsen, Elin
dc.contributor.authorKrossa, Sebastian
dc.contributor.authorBertilsson, Helena
dc.contributor.authorHeeren, Ron M.A.
dc.contributor.authorRye, Morten Beck
dc.contributor.authorGiskeødegård, Guro F.
dc.contributor.authorBathen, Tone Frost
dc.contributor.authorTessem, May-Britt
dc.date.accessioned2021-08-17T13:16:45Z
dc.date.available2021-08-17T13:16:45Z
dc.date.issued2021-01-29
dc.description.abstractBackground - Prostate cancer tissues are inherently heterogeneous, which presents a challenge for metabolic profiling using traditional bulk analysis methods that produce an averaged profile. The aim of this study was therefore to spatially detect metabolites and lipids on prostate tissue sections by using mass spectrometry imaging (MSI), a method that facilitates molecular imaging of heterogeneous tissue sections, which can subsequently be related to the histology of the same section.<p> <p>Methods - Here, we simultaneously obtained metabolic and lipidomic profiles in different prostate tissue types using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MSI. Both positive and negative ion mode were applied to analyze consecutive sections from 45 fresh-frozen human prostate tissue samples (N = 15 patients). Mass identification was performed with tandem MS.<p> <p>Results - Pairwise comparisons of cancer, non-cancer epithelium, and stroma revealed several metabolic differences between the tissue types. We detected increased levels of metabolites crucial for lipid metabolism in cancer, including metabolites involved in the carnitine shuttle, which facilitates fatty acid oxidation, and building blocks needed for lipid synthesis. Metabolites associated with healthy prostate functions, including citrate, aspartate, zinc, and spermine had lower levels in cancer compared to non-cancer epithelium. Profiling of stroma revealed higher levels of important energy metabolites, such as ADP, ATP, and glucose, and higher levels of the antioxidant taurine compared to cancer and non-cancer epithelium.<p> <p>Conclusions - This study shows that specific tissue compartments within prostate cancer samples have distinct metabolic profiles and pinpoint the advantage of methodology providing spatial information compared to bulk analysis. We identified several differential metabolites and lipids that have potential to be developed further as diagnostic and prognostic biomarkers for prostate cancer. Spatial and rapid detection of cancer-related analytes showcases MALDI-TOF MSI as a promising and innovative diagnostic tool for the clinic.en_US
dc.identifier.citationAndersen MK, Høiem TS, Claes BS, Balluff B, Marta, Richardsen ER, Krossa S, Bertilsson H, Heeren RM, Rye MB, Giskeødegård G.F., Bathen TF, Tessem MB. Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI. Cancer & Metabolism. 2021en_US
dc.identifier.cristinIDFRIDAID 1885033
dc.identifier.doihttps://doi.org/10.1186/s40170-021-00242-z
dc.identifier.issn2049-3002
dc.identifier.urihttps://hdl.handle.net/10037/22102
dc.language.isoengen_US
dc.publisherBMCen_US
dc.relation.journalCancer & Metabolism
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/ProstOmics/758306/Norway/'Tissue is the issue': a multi-omics approach to improve prostate cancer diagnosis//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleSpatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSIen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel