Show simple item record

dc.contributor.authorOpstad, Ida S.
dc.contributor.authorGodtliebsen, Gustav
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorMyrmel, Truls
dc.contributor.authorAgarwal, Krishna
dc.contributor.authorBirgisdottir, Åsa Birna
dc.date.accessioned2021-12-06T09:56:12Z
dc.date.available2021-12-06T09:56:12Z
dc.date.issued2021-11-12
dc.description.abstractMitochondria are essential energy-providing organelles of particular importance in energy-demanding tissue such as the heart. The production of mitochondria-derived vesicles (MDVs) is a cellular mechanism by which cells ensure a healthy pool of mitochondria. These vesicles are small and fast-moving objects not easily captured by imaging. In this work, we have tested the ability of the optical super-resolution technique 3DSIM to capture high-resolution images of MDVs. We optimized the imaging conditions both for high-speed video microscopy and fixed-cell imaging and analysis. From the 3DSIM videos, we observed an abundance of MDVs and many dynamic mitochondrial tubules. The density of MDVs in cells was compared for cells under normal growth conditions and cells during metabolic perturbation. Our results indicate a higher abundance of MDVs in H9c2 cells during glucose deprivation compared with cells under normal growth conditions. Furthermore, the results reveal a large untapped potential of 3DSIM in MDV research.en_US
dc.identifier.citationOpstad, Godtliebsen, Ahluwalia, Myrmel, Agarwal, Birgisdottir. Mitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopy. Journal of Biophotonics. 2021en_US
dc.identifier.cristinIDFRIDAID 1954475
dc.identifier.doi10.1002/jbio.202100305
dc.identifier.issn1864-063X
dc.identifier.issn1864-0648
dc.identifier.urihttps://hdl.handle.net/10037/23283
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.ispartofGodtliebsen, G. (2024). Exploring mitochondrial quality control mechanisms and mitochondria-lipid droplet interactions in cardiac cell models. (Doctoral thesis). <a href=https://hdl.handle.net/10037/32271>https://hdl.handle.net/10037/32271</a>
dc.relation.journalJournal of Biophotonics
dc.relation.projectIDHelse Nord RHF: HNF1449- 19en_US
dc.relation.projectIDUiT Norges arktiske universitet: 2061348en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Physics: 430en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Fysikk: 430en_US
dc.titleMitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopyen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record