Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay

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https://hdl.handle.net/10037/24203
DOI
https://doi.org/10.1007/978-1-0716-1016-9_5
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Date
2021-04-14
Type
Journal article
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Peer reviewed

Author
Nilsson, Per; Pettersen, Kristin; Oppermann, Martin; Skjeflo, Espen Waage; Fure, Hilde; Christiansen, Dorte; Mollnes, Tom Eirik
Abstract
Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.
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This version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at http://dx.doi.org/10.1007/978-1-0716-1016-9_5.
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Springer Nature
Citation
Nilsson P, Pettersen K, Oppermann, Skjeflo EW, Fure H, Christiansen D, Mollnes TE. Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay. Methods in molecular biology. 2021;2227:51-59
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