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dc.contributor.authorMelani, Mariana
dc.contributor.authorValko, Ayelen
dc.contributor.authorRomero, Nuria M
dc.contributor.authorAguilera, Milton O
dc.contributor.authorAcevedo, Julieta M
dc.contributor.authorBhujabal, Zambarlal
dc.contributor.authorPerez-Perri, Joel
dc.contributor.authorDe La Riva-Carrasco, Rocio V
dc.contributor.authorKatz, Maximiliano J
dc.contributor.authorSorianello, Eleonora
dc.contributor.authorD'Alessio, Cecilia
dc.contributor.authorJuhasz, Gabor
dc.contributor.authorJohansen, Terje
dc.contributor.authorColombo, Maria I
dc.contributor.authorWappner, Pablo
dc.date.accessioned2022-04-29T11:32:47Z
dc.date.available2022-04-29T11:32:47Z
dc.date.issued2017-09-13
dc.description.abstractAutophagy is an evolutionary conserved process by which eukaryotic cells undergo self-digestion of cytoplasmic components. Here we report that a novel Drosophila immunophilin, which we have named Zonda, is critically required for starvation-induced autophagy. We show that Zonda operates at early stages of the process, specifically for Vps34-mediated phosphatidylinositol 3-phosphate (PI3P) deposition. Zonda displays an even distribution under basal conditions and, soon after starvation, nucleates in endoplasmic reticulum–associated foci that colocalize with omegasome markers. Zonda nucleation depends on Atg1, Atg13, and Atg17 but does not require Vps34, Vps15, Atg6, or Atg14. Zonda interacts physically with Atg1 through its kinase domain, as well as with Atg6 and Vps34. We propose that Zonda is an early component of the autophagy cascade necessary for Vps34- dependent PI3P deposition and omegasome formation.en_US
dc.identifier.citationMelani, Valko, Romero, Aguilera, Acevedo, Bhujabal Z, Perez-Perri, De La Riva-Carrasco, Katz, Sorianello, D'Alessio, Juhasz G, Johansen T, Colombo MI, Wappner P. Zonda is a novel early component of the autophagy pathway in Drosophila. Molecular Biology of the Cell. 2017;28(22):3070-3081en_US
dc.identifier.cristinIDFRIDAID 1542620
dc.identifier.doi10.1091/mbc.E16-11-0767
dc.identifier.issn1059-1524
dc.identifier.issn1939-4586
dc.identifier.urihttps://hdl.handle.net/10037/24953
dc.language.isoengen_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.relation.journalMolecular Biology of the Cell
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2017 The Author(s)en_US
dc.titleZonda is a novel early component of the autophagy pathway in Drosophilaen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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