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dc.contributor.authorJøraholmen, May Wenche
dc.contributor.authorDamdimopoulou, Pauliina
dc.contributor.authorAcharya, Ganesh
dc.contributor.authorSkalko-Basnet, Natasa
dc.date.accessioned2022-06-21T09:20:40Z
dc.date.available2022-06-21T09:20:40Z
dc.date.issued2022-06-17
dc.description.abstractThe natural polyphenol resveratrol (RES) has shown great potential as an antimicrobial, including against microbes associated with vaginal infections. To fully exploit the activities of RES, an all-natural ingredients formulation for RES delivery at vaginal site has been developed, namely liposomes loaded with RES, incorporated into a chitosan hydrogel as secondary vehicle. Although considered non-toxic and safe on their own, the compatibility of the final formulation must be evaluated for its biocompatibility and non-irritancy to the vaginal mucosa. As a preclinical safety assessment, the impact of RES formulation on the tissue viability, the effect on barrier function and cell monolayer integrity, and cytotoxicity were evaluated using the cell-based vaginal tissue model, the EpiVaginal™ tissue. RES liposomes-in-hydrogel formulations neither affected the mitochondrial activity, nor the integrity of the cell monolayer in RES concentration up to 60 µg/mL. Moreover, the barrier function was maintained to a greater extent by RES in formulation, emphasizing the benefits of the delivery system. Additionally, none of the tested formulations expressed an increase in lactate dehydrogenase activity compared to the non-treated tissues. The evaluation of the RES delivery system suggests that it is non-irritant and biocompatible with vaginal tissue in vitro in the RES concentrations considered as therapeutic.en_US
dc.identifier.citationJøraholmen, M.W.; Damdimopoulou, P.; Acharya, G.; Škalko-Basnet, N. Toxicity Assessment of Resveratrol Liposomes-in-hydrogel Delivery System by EpiVaginalTM Tissue Model. Pharmaceutics 2022, 14, 1295.en_US
dc.identifier.cristinIDFRIDAID 2033530
dc.identifier.doihttps://doi.org/10.3390/pharmaceutics14061295
dc.identifier.issn1999-4923
dc.identifier.urihttps://hdl.handle.net/10037/25517
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalPharmaceutics
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleToxicity Assessment of Resveratrol Liposomes-in-Hydrogel Delivery System by EpiVaginal Tissue Modelen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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