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dc.contributor.authorRuntuvuori-Salmela, Anniina
dc.contributor.authorKunttu, Heidi M. T.
dc.contributor.authorLaanto, Elina
dc.contributor.authorde Freitas Almeida, Gabriel Magno
dc.contributor.authorMäkelä, Kati
dc.contributor.authorMiddelboe, Mathias
dc.contributor.authorSundberg, Lotta-Riina
dc.date.accessioned2022-08-25T08:31:13Z
dc.date.available2022-08-25T08:31:13Z
dc.date.issued2022-01-20
dc.description.abstractIntensive aquaculture conditions expose fish to bacterial infections, leading to significant financial losses, extensive antibiotic use and risk of antibiotic resistance in target bacteria. Flavobacterium columnare causes columnaris disease in aquaculture worldwide. To develop a bacteriophage-based control of columnaris disease, we isolated and characterized 126 F. columnare strains and 63 phages against F. columnare from Finland and Sweden in 2017. Bacterial isolates were virulent on rainbow trout (Oncorhynchus mykiss) and fell into four previously described genetic groups A, C, E and G, with genetic groups C and E being the most virulent. Phage host range studied against a collection of 227 bacterial isolates (from 2013 to 2017) demonstrated modular infection patterns based on host genetic group. Phages infected contemporary and previously isolated bacterial hosts, but bacteria isolated most recently were generally resistant to previously isolated phages. Despite large differences in geographical origin, isolation year or host range of the phages, whole-genome sequencing of 56 phages showed high level of genetic similarity to previously isolated F. columnare phages (Ficleduovirus, Myoviridae). Altogether, this phage collection demonstrates a potential for use in phage therapy.en_US
dc.identifier.citationRuntuvuori-Salmela, Kunttu, Laanto, de Freitas Almeida, Mäkelä, Middelboe, Sundberg. Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control. Environmental Microbiology. 2022;24(5):2404-2420en_US
dc.identifier.cristinIDFRIDAID 2024171
dc.identifier.doi10.1111/1462-2920.15901
dc.identifier.issn1462-2912
dc.identifier.issn1462-2920
dc.identifier.urihttps://hdl.handle.net/10037/26395
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalEnvironmental Microbiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titlePrevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen controlen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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