Vis enkel innførsel

dc.contributor.authorBargheet, Ahmed
dc.contributor.authorKlingenberg, Claus Andreas
dc.contributor.authorEsaiassen, Eirin
dc.contributor.authorHjerde, Erik
dc.contributor.authorCavanagh, Jorunn Pauline
dc.contributor.authorBengtsson-Palme, Johan
dc.contributor.authorPettersen, Veronika K.
dc.date.accessioned2023-08-18T08:47:34Z
dc.date.available2023-08-18T08:47:34Z
dc.date.issued2023-05-13
dc.description.abstractBackground - Gestational age (GA) and associated level of gastrointestinal tract maturation are major factors driving the initial gut microbiota composition in preterm infants. Besides, compared to term infants, premature infants often receive antibiotics to treat infections and probiotics to restore optimal gut microbiota. How GA, antibiotics, and probiotics modulate the microbiota’s core characteristics, gut resistome and mobilome, remains nascent.<p> <p>Methods - We analysed metagenomic data from a longitudinal observational study in six Norwegian neonatal intensive care units to describe the bacterial microbiota of infants of varying GA and receiving different treatments. The cohort consisted of probiotic-supplemented and antibiotic-exposed extremely preterm infants (n = 29), antibiotic-exposed very preterm (n = 25), antibiotic-unexposed very preterm (n = 8), and antibiotic-unexposed full-term (n = 10) infants. The stool samples were collected on days of life 7, 28, 120, and 365, and DNA extraction was followed by shotgun metagenome sequencing and bioinformatical analysis.<p> <p>Findings - The top predictors of microbiota maturation were hospitalisation length and GA. Probiotic administration rendered the gut microbiota and resistome of extremely preterm infants more alike to term infants on day 7 and ameliorated GA-driven loss of microbiota interconnectivity and stability. GA, hospitalisation, and both microbiota-modifying treatments (antibiotics and probiotics) contributed to an elevated carriage of mobile genetic elements in preterm infants compared to term controls. Finally, Escherichia coli was associated with the highest number of antibiotic-resistance genes, followed by Klebsiella pneumoniae and Klebsiella aerogenes.<p> <p>Interpretation - Prolonged hospitalisation, antibiotics, and probiotic intervention contribute to dynamic alterations in resistome and mobilome, gut microbiota characteristics relevant to infection risk.<p> <p>Funding - Odd-Berg Group, Northern Norway Regional Health Authority.en_US
dc.identifier.citationBargheet, Klingenberg, Esaiassen, Hjerde, Cavanagh, Bengtsson-Palme, Pettersen. Development of early life gut resistome and mobilome across gestational ages and microbiota-modifying treatments. EBioMedicine. 2023;92en_US
dc.identifier.cristinIDFRIDAID 2151471
dc.identifier.doi10.1016/j.ebiom.2023.104613
dc.identifier.issn2352-3964
dc.identifier.urihttps://hdl.handle.net/10037/30066
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalEBioMedicine
dc.relation.projectIDSigma2: NN8021Ken_US
dc.relation.projectIDTromsø forskningsstiftelse: 18_CANS_ASen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleDevelopment of early life gut resistome and mobilome across gestational ages and microbiota-modifying treatmentsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)