A new preparation method for preparation of liposomes-in-hydrogels primed for treatment of skin diseases

Abstract

<p>Liposomes are spherical vesicles that form spontaneously when phospholipids are dispersed in an aqueous medium. However, to get liposomes with a more unimodal size distribution, liposomes are usually processed further to reduced size and lammellarity, typically by extrusion, sonication or homogenization. <p>Liposome formulations for topical application needs a vehicle to assure the desirable retention and adhesion of the drug-loaded liposomes onto the skin, and for this, hydrogels are regarded as promising systems. The hydrogel “soluble beta-glucan” (SBG) has caught our attention since it also promotes wound healing on its own. <p>Dual asymmetric centrifuge (DAC) utilizes a unique combination of two contra rotating movements of the sample-holder, which results in shear forces that efficiently homogenize and gives a size reduction of liposomes. We have investigated the use of DAC for both use in liposome size reduction and for further mixing of liposomes into hydrogels.