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dc.contributor.authorPassantino, Letizia
dc.contributor.authorCorriero, Aldo
dc.contributor.authorPousis, Christo
dc.contributor.authorZupa, Rosa
dc.contributor.authorPerillo, Antonella
dc.contributor.authorSuperio, Joshua Lustracion
dc.contributor.authorSwain, Jaya Kumari
dc.contributor.authorFoss, Atle
dc.contributor.authorGalindo-Villegas, Jorge
dc.contributor.authorVentriglia, Gianluca
dc.date.accessioned2024-01-02T13:40:00Z
dc.date.available2024-01-02T13:40:00Z
dc.date.issued2024-11-26
dc.description.abstractThe lumpfish, Cyclopterus lumpus, holds significant promise as a candidate for large-scale aquaculture production, particularly in its role as a cleaner fish used to manage sea lice infestations in Atlantic salmon Salmo salar farming. Melanomacrophage centers (MMCs) represent polymorphic structures present in the hemolymphopoietic organs of various vertebrates, serving as a widely applicable histological indicator of the fish immune and health status. This study aims to investigate the histochemical characteristics of MMCs within lumpfish livers and to compare MMC density between hatchery-produced (farmed) and wild individuals. Liver samples were collected from 34 lumpfish and subjected to a range of staining techniques, including haematoxylin-eosin, Azan-Mallory's trichrome, Masson-Fontana, Perls-Van Geison, Mallory's hemofuscin, immunohistochemical detection of cytochrome P450 monooxygenase 1 A (CYP1A), and the terminal deoxynucleotidyl transferase-mediated d'UTP nick-end labelling (TUNEL) method. Hepatocytes from hatchery-produced males exhibited notably high lipid content. Additionally, cells showing positive staining with Masson-Fontana, likely associated with the monocyte/macrophage lineage, were identified. Furthermore, small MMCs containing melanin, lipofuscin-ceroids, and ferric ions were detected. While the density of single monocytes/macrophages was markedly higher in hatchery-produced males, no significant discrepancies in MMCs density were observed between wild and hatchery-produced fish, or between males and females of the same origin. The study also revealed the presence of necrotic foci, characterized by hypertrophic hepatocytes positive for both TUNEL and CYP1A staining. These hypertrophic hepatocytes displayed large lipid droplets and pycnotic nuclei, with hatchery-produced males showing a higher numerical density of such foci. In contrast to findings in other fish species, the study found that MMCs did not appear to serve as reliable markers of health status in lumpfish. This conclusion was reached as MMCs density did not exhibit a correlation with necrotic foci or hepatocyte lipid content.en_US
dc.identifier.citationPassantino, Corriero, Pousis, Zupa, Perillo, Superio, Swain, Foss, Galindo-Villegas, Ventriglia. Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state. Aquaculture. 2024;581en_US
dc.identifier.cristinIDFRIDAID 2217779
dc.identifier.doi10.1016/j.aquaculture.2023.740417
dc.identifier.issn0044-8486
dc.identifier.issn1873-5622
dc.identifier.urihttps://hdl.handle.net/10037/32285
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalAquaculture
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/817992/EU/ERA-NET Cofund on Blue Bioeconomy - Unlocking the potential of aquatic bioresources/BlueBio/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleHepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health stateen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)