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dc.contributor.authoral Rubaye, Mushtaq Talib Shawi
dc.contributor.authorJanice, Jessin
dc.contributor.authorBjørnholt, Jørgen
dc.contributor.authorLöhr, Iren Høyland
dc.contributor.authorSundsfjord, Arnfinn Ståle
dc.contributor.authorHegstad, Kristin
dc.date.accessioned2024-01-25T09:36:38Z
dc.date.available2024-01-25T09:36:38Z
dc.date.issued2023-12-27
dc.description.abstractObjectives - We aimed to characterize the vanE cluster and its genetic support in the first Norwegian vanE-type isolates and assess genetic relatedness to other vanE isolates.<p> <p>Methods - Two vanE-type vancomycin resistant Enterococcus faecalis (vanE-VREfs) isolates (E1 and E2) recovered from the same patient 30 months apart were examined for antimicrobial susceptibility, genome sequence, vancomycin resistance induction, vanE transferability, genome mutation rate, and phylogenetic relationship to E. faecalis closed genomes and two vanE-VREfs from North America.<p> <p>Results - The ST34 E1 and E2 strains expressed low-level vancomycin resistance and susceptibility to teicoplanin. Their vanE gene clusters were part of a non-transferable Tn6202. The histidine kinase part of vanSE was expressed although a premature stop codon (E1) and insertion of a transposase (E2) truncated their vanSE gene. The vancomycin resistance phenotype in E1 was inducible while constitutive in E2. E1 showed a 125-fold higher mutation rate than E2. Variant calling showed 60 variants but nearly identical chromosomal gene content and synteny between the isolates. Their genomes also showed high similarity to another ST34 vanE-VREfs from Canada.<p> <p>Conclusion - In-depth genomic analyses of the first two vanE-VREfs found in Europe identified a single chromosomal insertion site of two variants of vanE-conferring Tn6202. Single nucleotide polymorphism (SNP) and core genome multilocus sequence type (cgMLST) analyses show the genomes are different. This can be explained by the high mutation rate of E1 and acquisition of different mobile genetic elements; thus, we believe the two isolates from the same patient are genetically related. Genome similarities also suggest relatedness between the Canadian and Norwegian vanE-VREfs.en_US
dc.identifier.citational Rubaye MTS, Janice JJ, Bjørnholt J, Löhr IH, Sundsfjord A, Hegstad K. The first vanE-type vancomycin resistant Enterococcus faecalis isolates in Norway – phenotypic and molecular characteristics. Journal of Global Antimicrobial Resistance. 2023;36:193-199en_US
dc.identifier.cristinIDFRIDAID 2222069
dc.identifier.doi10.1016/j.jgar.2023.12.021
dc.identifier.issn2213-7165
dc.identifier.issn2213-7173
dc.identifier.urihttps://hdl.handle.net/10037/32733
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalJournal of Global Antimicrobial Resistance
dc.relation.projectIDHelse Nord RHF: HNF1362-17en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleThe first vanE-type vancomycin resistant Enterococcus faecalis isolates in Norway – phenotypic and molecular characteristicsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)