dc.contributor.author | Hadler-Olsen, Elin Synnøve | |
dc.contributor.author | Winberg, Jan-Olof | |
dc.contributor.author | Reinholt, Finn P. | |
dc.contributor.author | Larsen, Terje | |
dc.contributor.author | Uhlin-Hansen, Lars | |
dc.contributor.author | Jenssen, Trond Geir | |
dc.contributor.author | Berg, Eli | |
dc.contributor.author | Kolset, Svein Olav | |
dc.date.accessioned | 2012-03-07T12:14:33Z | |
dc.date.available | 2012-03-07T12:14:33Z | |
dc.date.issued | 2011 | |
dc.description.abstract | Db/db mice are overweight, dyslipidemic and develop diabetic complications, relevant for similar complications in human type 2 diabetes. We have used db/db and db/+ control mice to investigate alterations in proteinase expression and activity in circulation and kidneys by SDS-PAGE zymography, electron microscopy, immunohistochemistry, Western blotting, and in situ zymography. Plasma from db/db mice contained larger amounts of serine proteinases compared to db/+ mice. Kidneys from the db/db mice had a significantly larger glomerular surface area and somewhat thicker glomerular basement membranes compared to the db/+ mice. Furthermore, kidney extracts from db/+ mice contained metalloproteinases with Mr of approximately 92000, compatible with MMP-9, not observed in db/db mice. These results indicate that higher levels of serine proteinases in plasma may serve as potential markers for kidney changes in db/db mice, whereas a decrease in MMP-9 in the kidney may be related to the glomerular changes. | en |
dc.identifier.citation | ISRN Endocrinology (2011), Article ID 832642, 10 pages | en |
dc.identifier.cristinID | FRIDAID 845725 | |
dc.identifier.doi | doi: 10.5402/2011/832642 | |
dc.identifier.issn | 2090-4630 | |
dc.identifier.uri | https://hdl.handle.net/10037/3913 | |
dc.identifier.urn | URN:NBN:no-uit_munin_3635 | |
dc.language.iso | eng | en |
dc.rights.accessRights | openAccess | |
dc.subject | VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical biochemistry: 726 | en |
dc.subject | VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk biokjemi: 726 | en |
dc.title | Proteases in plasma and kidney of db /db mice as markers of diabetes-induced nephropathy | en |
dc.type | Journal article | en |
dc.type | Tidsskriftartikkel | en |
dc.type | Peer reviewed | en |