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dc.contributor.authorHaugen, Peik
dc.contributor.authorWikmark, Odd-Gunnar
dc.contributor.authorVader, Anna
dc.contributor.authorCoucheron, Dag H.
dc.contributor.authorSjøttem, Eva
dc.contributor.authorJohansen, Steinar D.
dc.date.accessioned2012-04-23T10:29:30Z
dc.date.available2012-04-23T10:29:30Z
dc.date.issued2005
dc.description.abstractThe myxomycete Didymium iridis (isolate Panama 2) contains a mobile group I intron named Dir.S956-1 after position 956 in the nuclear small subunit (SSU) rRNA gene. The intron is efficiently spread through homing by the intron-encoded homing endonuclease I-DirI. Homing endonuclease genes (HEGs) usually spread with their associated introns as a unit, but infrequently also spread independent of introns (or inteins). Clear examples of HEG mobility are however sparse. Here, we provide evidence for the transfer of a HEG into a group I intron named Dir.S956-2 that is inserted into the SSU rDNA of the Costa Rica 8 isolate of D.iridis. Similarities between intron sequences that flank the HEG and rDNA sequences that flank the intron (the homing endonuclease recognition sequence) suggest that the HEG invaded the intron during the recent evolution in a homing-like event. Dir.S956-2 is inserted into the same SSU site as Dir.S956-1. Remarkably, the two group I introns encode distantly related splicing ribozymes with phylogenetically related HEGs inserted on the opposite strands of different peripheral loop regions. The HEGs are both interrupted by small spliceosomal introns that must be removed duringRNAmaturation.en
dc.identifier.citationNucleic Acids Research 33(2005) nr. 8 s. 2734-2741en
dc.identifier.cristinIDFRIDAID 677993
dc.identifier.doidoi: 10.1093/nar/gki564
dc.identifier.issn0305-1048
dc.identifier.urihttps://hdl.handle.net/10037/4128
dc.identifier.urnURN:NBN:no-uit_munin_3848
dc.language.isoengen
dc.publisherOxford University Pressen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical molecular biology: 711en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical genetics: 714en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714en
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715en
dc.title.alternativeThe recent transfer of a homing endonuclease geneen
dc.titleThe recent transfer of a homing endonuclease geneen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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