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dc.contributor.authorKarlsen, Christian
dc.contributor.authorEspelid, sigrun
dc.contributor.authorWillassen, Nils P
dc.contributor.authorPaulsen, steinar
dc.date.accessioned2013-12-09T08:56:18Z
dc.date.available2015-02-01T06:50:04Z
dc.date.issued2011
dc.description.abstractAliivibrio salmonicida is the aetiological agent of cold water vibriosis affecting farmed fish species, a disease that today is fully controlled by vaccination. However, the molecular mechanisms behind the successful vaccine are largely unknown. In order to gain insight into the possible mechanisms of A. salmonicida vaccines, we report here the profiles of both the outer membrane and secreted subproteomes of A. salmonicida LFI315. The 2 subproteomes were resolved by 2-dimensional electrophoresis that identified a total of 82 protein entries. Monoclonal antibodies specific to an unidentified protein antigen were utilized in the immunoproteomic analysis of both outer membrane proteins and extracellular proteins. The immunogenic protein was located in both subproteomes and identified as a 20 kDa peptidoglycan-associated lipoprotein (Pal). The identity of the antigen was verified by heterologous expression of the cloned A. salmonicida pal gene (VSAL_I1899). It is likely that the immunogenic Pal-like protein is among the constituents that act as a protective antigen in the successful vaccine used today. In view of this, it may be considered a potentially useful component in future vaccine development and pathogenicity studies.en
dc.identifier.citationDiseases of Aquatic Organisms 93(2011) nr. 3 s. 215-223en
dc.identifier.cristinIDFRIDAID 830490
dc.identifier.doihttp://dx.doi.org/10.3354/dao02302
dc.identifier.issn0177-5103
dc.identifier.urihttps://hdl.handle.net/10037/5588
dc.identifier.urnURN:NBN:no-uit_munin_5287
dc.language.isoengen
dc.publisherInter-Research (IR)en
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497en
dc.titleIdentification and cloning of immunogenic Aliivibrio salmonicida Pal-like protein present in profiled outer membrane and secreted subproteomeen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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