Fluorescence Microscopy is still the most preferred tool for studying the cell's inner structures. One of the reasons for this preference is its great selectivity, which allows it to label specific types of structures and visualize them with high contrast. However, its resolution is conventionally limited due to the diffraction of light, which makes the study of cells at scales below 200 nm challenging. ...

The influence of fluorinated substituents on the luminescent properties of rhenium-oxo, osmium-nitrido, and gold triarylcorroles was studied via a comparison of four ligands: triphenylcorrole (TPC), tris(ptrifluoromethylphenyl)corrole (TpCF₃PC), tris{3,5-bis(trifluoromethyl)- phenyl}corrole (T3,5-CF₃PC), and tris(pentafluorophenyl)corrole (TPFPC). For each metal series ...

This thesis explores the prospect of a waveguide device for optical trapping and Raman spectroscopy of single biological nanoparticles. The aim of the work is to develop a Raman-on-chip device capable of training and measuring single particles in individual trapping sites, such that the throughput of the device can be increased through parallelization. The challenge posed by the induced Raman ...

Superoxide dismutases (SODs) are enzymes that catalyze the dismutation of the superoxide radical anion into O² and H²O² in a two-step reaction. They are ubiquitous to all forms of life and four different types of metal centers are detected, dividing this class of enzymes into Cu-/Zn-, Ni-, Mn-, and Fe-SODs. In this study, a superoxide dismutase from the ...

Abstract The reliable operation of monoblock centrifugal pumps (MCP) is crucial in various industrial applications. Achieving optimal performance and minimizing costly downtime requires effectively detecting and diagnosing faults in critical pump components. This study proposes an innovative approach that leverages deep transfer learning techniques. An accelerometer was adopted to capture vibration ...

Land use is a major threat to terrestrial biodiversity. Life cycle assessment is a tool that can assess such threats and thereby support environmental decision-making. Within the Global Guidance for Life Cycle Impact Assessment (GLAM) project, the Life Cycle Initiative hosted by UN Environment aims to create a life cycle impact assessment method across multiple impact categories, including ...

Background: Individuals with diabetes rely on medical equipment (eg, continuous glucose monitoring (CGM), hybrid closed-loop systems) and mobile applications to manage their condition, providing valuable data to health care providers. Data sharing from this equipment is regulated via Terms of Service (ToS) and Privacy Policy documents. The introduction of the Medical Devices Regulation (MDR) and In ...

The extremely radiation and desiccation resistant bacterium Deinococcus radiodurans possesses three genes encoding Endonuclease III-like enzymes (DrEndoIII1, DrEndoIII2, DrEndoIII3). In vitro enzymatic activity measurements revealed that DrEndoIII2 is the main Endonuclease III in this organism, while DrEndoIII1 and 3 possess unusual and, so far, no detectable EndoIII activity, respectively. In ...

Convolutional neural networks (CNNs) have been extensively utilized in medical image processing to automatically extract meaningful features and classify various medical conditions, enabling faster and more accurate diagnoses. In this paper, LeNet, a classic CNN architecture, has been successfully applied to breast cancer data analysis. It demonstrates its ability to extract discriminative ...

Raman spectroscopy can give a chemical ’fingerprint’ from both inorganic and organic samples, and has become a viable method of measuring the chemical composition of single biological particles. In parallel, integration of waveguides and microfluidics allows for the creation of miniaturized optical sensors in lab-on-a-chip devices. The prospect of combining integrated optics and Raman spectroscopy ...