Subburaj, Saminathan; Zanatta, Caroline Bedin; Nunn, Jennifer Ann Lillebo; Hoepers, Aline Martins; Nodari, Rubens Onofre; Agapito-Tenfen, Sarah Zanon (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-07-12)
CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform based on the direct delivery of Cas9 RNPs to soybean protoplasts for genetic screens in knockout gene-edited soybean lines without the transfection of DNA vectors. The platform ...