Identification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract
Permanent link
https://hdl.handle.net/10037/6019Date
2013Type
Journal articleTidsskriftartikkel
Peer reviewed
Author
Korup, Sarah; Rietscher, Janita; Calvignac-Spencer, Sébastien; Trusch, Franziska; Hofmann, Jörg; Moens, Ugo; Sauer, Igor; Voigt, Sebastien; Schmuck, Rosa; Ehlers, BernhardAbstract
Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic
polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected
human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode
large and small T antigens and the 3 structural proteins VP1, VP2 and VP3. Phylogenetic analyses did not reveal a close
relationship to any known human or animal polyomavirus. Investigation of organs, body fluids and excretions of diseased
individuals and healthy subjects with both HPyV12-specific nested PCR and quantitative real-time PCR revealed additional
virus-positive samples of resected liver, cecum and rectum tissues and a positive fecal sample. A capsomer-based IgG ELISA
was established using the major capsid protein VP1 of HPyV12. Seroprevalences of 23% and 17%, respectively, were
determined in sera from healthy adults and adolescents and a pediatric group of children. These data indicate that the virus
naturally infects humans and that primary infection may already occur in childhood.
Publisher
Public Library of Science (PLoS)Citation
PLoS ONE (2013), vol. 8(3): e58021.Metadata
Show full item recordCollections
The following license file are associated with this item:
Related items
Showing items related by title, author, creator and subject.
-
Prognostic Impacts of Angiopoietins in NSCLC Tumor Cells and Stroma : VEGF-A Impact Is Strongly Associated with Ang-2
Andersen, Sigve; Dønnem, Tom; Al-Shibli, Khalid Ibrahim; Al-Saad, Samer; Stenvold, Helge; Busund, Lill-Tove; Bremnes, Roy M. (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)Angiopoietins and their receptor Tie-2 are, in concert with VEGF-A, key mediators in angiogenesis. This study evaluates the prognostic impact of all known human angiopoietins (Ang-1, Ang-2 and Ang-4) and their receptor Tie-2, as well as their relation to the prognostic expression of VEGF-A. 335 unselected stage I-IIIA NSCLC-patients were included and tissue samples of respective tumor cells and ... -
The Temporomandibular Joint in Juvenile Idiopathic Arthritis, focusing on Quality of Life, Oral Microbiome and Intervention
Frid, Paula (Doctoral thesis; Doktorgradsavhandling, 2020-10-02)The temporomandibular joint (TMJ) is commonly involved in juvenile idiopathic arthritis (JIA), and may lead to impaired mouth opening, pain and facial growth disturbances. Asymptomatic TMJ arthritis may be diagnosed late in the disease course, thus management is challenging. The overall objectives of this thesis were to provide new knowledge on quality of life (QoL), the oral microbiome and interventions ... -
Humant papillomavirus : en litteraturstudie om HPV, dets relasjon til cancer og tiltak mot videre spredning av virus
Gabrielsen, Endre (Master thesis; Mastergradsoppgave, 2012-06-01)I 1983 oppdaget zur Hausen sammenhengen mellom Humant Papillomavirus (HPV) og livmorhalskreft. På denne tiden visste man ikke at det var HPV som var årsaken til at Helaceller kunne leve in vitro. Ny forskning relaterer HPV til en rekke andre cancertyper. En stor andel anal-, oropharyngeal-, penis-, vaginal-, og vulvacancer skyldes HPV. Det er også påvist HPV i tumorvev fra øsofagus, larynx, lunge, ...