Show simple item record

dc.contributor.authorSobhkhez, Mehrdad
dc.contributor.authorSkjesol, Astrid
dc.contributor.authorThomassen, Ernst Ivan
dc.contributor.authorIliev, Dimitar Borisov
dc.contributor.authorSun, Baojian
dc.contributor.authorRobertsen, Børre
dc.contributor.authorJørgensen, Jorunn B
dc.date.accessioned2014-11-07T12:23:01Z
dc.date.available2014-11-07T12:23:01Z
dc.date.issued2014
dc.description.abstractMammalian IRF9 and STAT2, together with STAT1, form the ISGF3 transcription factor complex, which is critical for type I interferon (IFN)-induced signaling, while IFNγ stimulation is mediated by homodimeric STAT1 protein. Teleost fish are known to possess most JAK and STAT family members, however, description of their functional activity in lower vertebrates is still scarce. In the present study we have identified two different STAT2 homologs and one IRF9 homolog from Atlantic salmon (Salmo salar). Both proteins have domain-like structures with functional motifs that are similar to higher vertebrates, suggesting that they are orthologs to mammalian STAT2 and IRF9. The two identified salmon STAT2s, named STAT2a and STAT2b, showed high sequence identity but were divergent in their transactivation domain (TAD). Like STAT1, ectopically expressed STAT2a and b were shown to be tyrosine phosphorylated by type I IFNs and, interestingly, also by IFNγ. Microscopy analyses demonstrated that STAT2 co-localized with STAT1a in the cytoplasm of unstimulated cells, while IFNa1 and IFNγ stimulation seemed to favor their nuclear localization. Overexpression of STAT2a or STAT2b together with STAT1a activated a GAS-containing reporter gene construct in IFNγ-stimulated cells. The highest induction of GAS promoter activation was found in IFNγ-stimulated cells transfected with IRF9 alone. Taken together, these data suggest that salmon STAT2 and IRF9 may have a role in IFNγ-induced signaling and promote the expression of GAS-driven genes in bony fish. Since mammalian STAT2 is primarily an ISGF3 component and not involved in IFNγ signaling, our finding features a novel role for STAT2 in fish.en
dc.identifier.citationFEBS Open Bio 4(2014) s. 858-871en
dc.identifier.cristinIDFRIDAID 1167898
dc.identifier.doiDOI: 10.1016/j.fob.2014.09.007
dc.identifier.doihttp://dx.doi.org/10.1016/j.fob.2014.09.007
dc.identifier.issn2211-5463
dc.identifier.urihttps://hdl.handle.net/10037/6805
dc.identifier.urnURN:NBN:no-uit_munin_6404
dc.language.isoengen
dc.publisherElsevieren
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497en
dc.subjectAtlantic salmonen
dc.subjectSTAT2en
dc.subjectIRF9en
dc.subjectIFNen
dc.subjectFishen
dc.titleStructural and functional characterization of salmon STAT1, STAT2 and IRF9 homologs sheds light on interferon signaling in teleostsen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


File(s) in this item

Thumbnail
Thumbnail

This item appears in the following collection(s)

Show simple item record