The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells
ForfatterAskarian, Fatemeh; Ajayi, Clement; Hanssen, Anne-Merethe; Van Sorge, Nina M.; Pettersen, Ingvild; Diep, Dzung Bao; Sollid, Johanna U. Ericson; Johannessen, Mona
Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors involved in colonization are adhesins such as the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1) as a novel interaction partner for SdrD. Genetic deletion of sdrD in S. aureusNCTC8325-4 through allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells in vitro. Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells resulted in increased adherence of S. aureus NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4ΔsdrD, to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface. These data demonstrate that Dsg1 is a host ligand for SdrD.
Source at http://doi.org/10.1038/srep22134
Er en del avAjayi, C. (2018). Determinants of Staphylococcus aureus Colonization and Infection. “Exploring the Role of Cell Wall Anchored Proteins in Adhesion and Immune Evasion”. Doctoral thesis. http://hdl.handle.net/10037/14070
ForlagNature Publishing Group
SiteringAskarian, F., Ajayi, C., Hanssen, A-M., van Sorge, N.M., Pettersen, I., Diep, D.B. ... Johannessen, M. (2016). The interaction beween Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells. Scientific Reports, 6, 22134. http://doi.org/10.1038/srep22134
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