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dc.contributor.authorWagner, Theresa
dc.contributor.authorJoshi, Bishnu
dc.contributor.authorJames Peter, Jessin Janice
dc.contributor.authorAskarian, Fatemeh
dc.contributor.authorSkalko-Basnet, Natasa
dc.contributor.authorHagestad, Ole Christian
dc.contributor.authorMekhlif, Ahmed
dc.contributor.authorWai, Sun Nyunt
dc.contributor.authorHegstad, Kristin
dc.contributor.authorJohannessen, Mona
dc.date.accessioned2019-01-31T22:18:44Z
dc.date.available2019-01-31T22:18:44Z
dc.date.issued2018-05-30
dc.description.abstract<i>Enterococcus faecium</i> is a commensal but also a bacteremia causing pathogen, which is inherently resistant to several antimicrobials and has a great ability to acquire new traits. Bacterial membrane vesicles (MVs) are increasingly recognized as a mode of cell-free communication and a way to deliver virulence factors and/or antimicrobial resistance determinants. These features make MVs interesting research targets in research on critical hospital pathogens. This study describes for the first time that <i>E. faecium</i> strains produce MVs. It presents a morphological as well as a proteomic analysis of MVs isolated from four different, clinically relevant <i>E. faecium</i> strains grown under two different conditions and identifies MV-associated proteins in all of them. Interestingly, 11 virulence factors are found among the MV-associated proteins, including biofilm-promoting proteins and extracellular matrix-binding proteins, which may aid in enterococcal colonization. Additionally, 11 antimicrobial resistance-related proteins were MV-associated. Among those, all proteins encoded by the vanA-cluster of a vancomycin resistant strain were found to be MV-associated. This implies that <i>E. faecium</i> MVs may be utilized by the bacterium to release proteins promoting virulence, pathogenicity and antimicrobial resistance.<br><br> <i>Significance -</i> Enterococcal infections, especially bacteremia and endocarditis, are challenging to treat because <i>E. faecium</i> have acquired resistance to multiple classes of antimicrobials, including ampicillin, aminoglycosides, and glycopeptides. Thus, research on different modes of enterococcal pathogenicity is warranted. This study utilized a proteomic approach to identify MV-associated proteins of different nosocomial <i>E. faecium</i> strains representing four clinically relevant sequence types (STs), namely ST17, ST18, ST78, and ST192. The presented data suggest that <i>E. faecium</i> MVs are involved in virulence and antimicrobial resistance.en_US
dc.description.sponsorshipNorthern Norway Regional Health Authority Medical Research Programmeen_US
dc.identifier.citationWagner, T., Joshi, B., Janice, J., Askarian, F., Skalko-Basnet, N., Hagestad, O.C., ... Johannessen M. (2018). Enterococcus faecium produces membrane vesicles containing virulence factors and antimicrobial resistance related proteins. <i>Journal of Proteomics, 187</i>, 28-38. https://doi.org/10.1016/j.jprot.2018.05.017en_US
dc.identifier.cristinIDFRIDAID 1591458
dc.identifier.doi10.1016/j.jprot.2018.05.017
dc.identifier.issn1874-3919
dc.identifier.issn1876-7737
dc.identifier.urihttps://hdl.handle.net/10037/14580
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofJoshi, B. (2021). Bacterial Extracellular vesicles and their cargo. (Doctoral thesis). <a href=https://hdl.handle.net/10037/20169>https://hdl.handle.net/10037/20169 </a>
dc.relation.journalJournal of Proteomics
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FORSKSKOLE/ 249062/Norway/Norwegian Research School in Infection Biology and Antimicrobials/IBA/en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776en_US
dc.subjectMembrane vesiclesen_US
dc.subjectProteomicsen_US
dc.subjectVirulence factorsen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectEnterococcusen_US
dc.subjectE. faeciumen_US
dc.titleEnterococcus faecium produces membrane vesicles containing virulence factors and antimicrobial resistance related proteinsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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