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dc.contributor.authorLellahi, Seyed Mohammad
dc.contributor.authorRosenlund, Ingrid Arctander
dc.contributor.authorHedberg, Annica
dc.contributor.authorKiær, Liv Torill
dc.contributor.authorMikkola, Ingvild
dc.contributor.authorKnutsen, Erik
dc.contributor.authorPerander, Maria
dc.date.accessioned2019-06-26T12:59:56Z
dc.date.available2019-06-26T12:59:56Z
dc.date.issued2018-10-10
dc.description.abstractThe long noncoding RNA (lncRNA) <i>NEAT1</i> (nuclear enriched abundant transcript 1) is the architectural component of nuclear paraspeckles, and it has recently gained considerable attention as it is abnormally expressed in pathological conditions such as cancer and neurodegenerative diseases. <i>NEAT1</i> and paraspeckle formation are increased in cells upon exposure to a variety of environmental stressors and believed to play an important role in cell survival. The present study was undertaken to further investigate the role of <i>NEAT1</i> in cellular stress response pathways. We show that <i>NEAT1</i> is a novel target gene of heat shock transcription factor 1 (HSF1) and is up-regulated when the heat shock response pathway is activated by sulforaphane (SFN) or elevated temperature. HSF1 binds specifically to a newly identified conserved heat shock element in the <i>NEAT1</i> promoter. In line with this, SFN induced the formation of <i>NEAT1</i>-containing paraspeckles via an HSF1-dependent mechanism. HSF1 plays a key role in the cellular response to proteotoxic stress by promoting the expression of a series of genes, including those encoding molecular chaperones. We have found that the expression of HSP70, HSP90, and HSP27 is amplified and sustained during heat shock in <i>NEAT1</i>-depleted cells compared with control cells, indicating that <i>NEAT1</i> feeds back via an unknown mechanism to regulate HSF1 activity. This interrelationship is potentially significant in human diseases such as cancer and neurodegenerative disorders.en_US
dc.description.sponsorshipNorthern Norway Regional Health Authorityen_US
dc.descriptionThis research was originally published in the <i>Journal of Biological Chemistry, 293</i>(49), 18965–18976. © the American Society for Biochemistry and Molecular Biology or © the Author(s). <a href=http://www.jbc.org/site/misc/edpolicy.xhtml#copyright>Terms and conditions</a> apply.en_US
dc.identifier.citationLellahi, S.M., Rosenlund, I.A., Hedberg, A., Kiær, L.T., Mikkola, I., Knutsen, E. & Perander, M. (2018). The long noncoding RNA NEAT1 and nuclear paraspeckles are up-regulated by the transcription factor HSF1 in the heat shock response. <i>Journal of Biological Chemistry, 293</i>(49), 18965–18976. https://doi.org/10.1074/jbc.RA118.004473en_US
dc.identifier.cristinIDFRIDAID 1638721
dc.identifier.doi10.1074/jbc.RA118.004473
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.urihttps://hdl.handle.net/10037/15610
dc.language.isoengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.relation.ispartofLellahi, S.M. (2019). Breast cancer-associated NEAT1 in cellular stress response pathways. (Doctoral thesis). <a href=https://hdl.handle.net/10037/15809>https://hdl.handle.net/10037/15809</a>.
dc.relation.journalJournal of Biological Chemistry
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en_US
dc.subjectlong noncoding RNA (long ncRNA, lncRNA)en_US
dc.subjectheat shock factor protein 1 (HSF1)en_US
dc.subjectheat shock protein (HSP)en_US
dc.subjectgene expressionen_US
dc.subjecttranscription regulationen_US
dc.subjectheat shock responseen_US
dc.subjectNEAT1en_US
dc.subjectparaspeckleen_US
dc.subjectsulforaphaneen_US
dc.titleThe long noncoding RNA NEAT1 and nuclear paraspeckles are up-regulated by the transcription factor HSF1 in the heat shock responseen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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