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dc.contributor.authorSolbakken, Monica Hongrø
dc.contributor.authorJentoft, Sissel
dc.contributor.authorReitan, Trond
dc.contributor.authorMikkelsen, Helene
dc.contributor.authorJakobsen, Kjetill Sigurd
dc.contributor.authorSeppola, Marit
dc.date.accessioned2020-01-14T07:33:55Z
dc.date.available2020-01-14T07:33:55Z
dc.date.issued2019-05-28
dc.description.abstractAtlantic cod has lost the Major Histocompatibility complex class II pathway – central to pathogen presentation, humoral response and immunity. Here, we investigate the immunological response of Atlantic cod subsequent to dip vaccination with <i>Vibrio anguillarum</i> bacterin using transcriptome sequencing. The experiment was conducted on siblings from an Atlantic cod family found to be highly susceptible towards vibriosis where vaccination has demonstrated improved pathogen resistance. Gene expression analyses at 2, 4, 21 and 42 days post vaccination revealed GO-term enrichment for muscle, neuron and metabolism-related pathways. In-depth characterization of immune-related GO terms demonstrated down-regulation of MHCI antigen presentation, C-type lectin receptor signaling and granulocyte activation over time. Phagocytosis, interferon-gamma signaling and negative regulation of innate immunity were increasingly up-regulated over time. Individual differentially expressed immune genes implies weak initiation of acute phase proteins with little or no inflammation. Furthermore, gene expression indicates presence of T-cells, NK-like cells, B-cells and monocytes/macrophages. Three <i>MHCI</i> transcripts were up-regulated with <i>B2M</i> and <i>SEC61</i>. Overall, we find no clear immune-related transcriptomic response which could be attributed to Atlantic cod's alternative immune system. However, we cannot rule out that this response is related to vaccination protocol/sampling strategy. Earlier functional studies demonstrate significant memory in Atlantic cod post dip vaccination and combined with our results indicate the presence of other adaptive immunity mechanisms. In particular, we suggest that further investigations should look into CD8+ memory T-cells, γδ T-cells, T-cell independent memory or memory induced through NK-like/other lymphoid cells locally in the mucosal lining for this particular vaccination strategy.en_US
dc.identifier.citationSolbakken MH, Jentoft S, Reitan T, Mikkelsen HV, Jakobsen KS, Seppola M. Whole transcriptome analysis of the Atlantic cod vaccine response reveals subtle changes in adaptive immunity. Comparative Biochemistry and Physiology - Part D:Genomics and Proteomics. 2019;31:100597:1-12en_US
dc.identifier.cristinIDFRIDAID 1710443
dc.identifier.doi10.1016/j.cbd.2019.100597
dc.identifier.issn1744-117X
dc.identifier.issn1878-0407
dc.identifier.urihttps://hdl.handle.net/10037/17087
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalComparative Biochemistry and Physiology - Part D:Genomics and Proteomics
dc.relation.projectIDNorges forskningsråd: 199672en_US
dc.relation.projectIDNorges forskningsråd: 222378en_US
dc.relation.projectIDNorges forskningsråd: 199806en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/199806/Norway/Translating the cod genome for aquaculture//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/222378/Norway/Functional and comparative immunology of a teleosts world without MHC II//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUKS/199672/Norway/Intracellular lifestyle of Francisella piscicida in Atlantic cod//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923en_US
dc.titleWhole transcriptome analysis of the Atlantic cod vaccine response reveals subtle changes in adaptive immunityen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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