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dc.contributor.authorWirth, Martina
dc.contributor.authorZhang, Wenxin
dc.contributor.authorRazi, Minoo
dc.contributor.authorNyoni, Lynet
dc.contributor.authorJoshi, Dhira
dc.contributor.authorO'Reilly, Nicola
dc.contributor.authorJohansen, Terje
dc.contributor.authorTooze, Sharon
dc.contributor.authorMouilleron, Stephane
dc.date.accessioned2020-01-14T11:10:45Z
dc.date.available2020-01-14T11:10:45Z
dc.date.issued2019-05-03
dc.description.abstractAutophagy is an essential recycling and quality control pathway. Mammalian ATG8 proteins drive autophagosome formation and selective removal of protein aggregates and organelles by recruiting autophagy receptors and adaptors that contain a LC3-interacting region (LIR) motif. LIR motifs can be highly selective for ATG8 subfamily proteins (LC3s/GABARAPs), however the molecular determinants regulating these selective interactions remain elusive. Here we show that residues within the core LIR motif and adjacent C-terminal region as well as ATG8 subfamily-specific residues in the LIR docking site are critical for binding of receptors and adaptors to GABARAPs. Moreover, rendering GABARAP more LC3B-like impairs autophagy receptor degradation. Modulating LIR binding specificity of the centriolar satellite protein PCM1, implicated in autophagy and centrosomal function, alters its dynamics in cells. Our data provides new mechanistic insight into how selective binding of LIR motifs to GABARAPs is achieved, and elucidate the overlapping and distinct functions of ATG8 subfamily proteins.en_US
dc.identifier.citationWirth M, Zhang W, Razi, Nyoni, Joshi, O'Reilly N, Johansen T, Sharon, Mouilleron S. Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins. Nature Communications. 2019;10(1):2055en_US
dc.identifier.cristinIDFRIDAID 1771329
dc.identifier.doi10.1038/s41467-019-10059-6
dc.identifier.issn2041-1723
dc.identifier.urihttps://hdl.handle.net/10037/17090
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.relation.journalNature Communications
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/214448/Norway/Selective autophagy and cell signalling: Regulation of p62/SQSTM1 and functional studies of novel LIR-containing proteins//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/249884/Norway/Autophagy-regulated Signalosomes in Cellular Stress and Disease Pathways//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleMolecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteinsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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