ub.xmlui.mirage2.page-structure.muninLogoub.xmlui.mirage2.page-structure.openResearchArchiveLogo
    • EnglishEnglish
    • norsknorsk
  • Velg spraakEnglish 
    • EnglishEnglish
    • norsknorsk
  • Administration/UB
View Item 
  •   Home
  • Det helsevitenskapelige fakultet
  • Institutt for medisinsk biologi
  • Artikler, rapporter og annet (medisinsk biologi)
  • View Item
  •   Home
  • Det helsevitenskapelige fakultet
  • Institutt for medisinsk biologi
  • Artikler, rapporter og annet (medisinsk biologi)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

A Novel Truncated Form of Nephronectin Is Present in Small Extracellular Vesicles Isolated from 66cl4 Cells

Permanent link
https://hdl.handle.net/10037/17155
DOI
https://doi.org/10.1021/acs.jproteome.8b00859
Thumbnail
View/Open
article.pdf (6.673Mb)
Published version (PDF)
Date
2019-02-01
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Toraskar, Jimita Prashant; Norvoll Magnussen, Synnøve; Hagen, Lars; Sharma, Animesh; Hoang, Linh; Bjørkøy, Geir; Svineng, Gunbjørg; Steigedal, Tonje S.
Abstract
Extracellular vesicles are emerging as biomarkers in breast cancer. Our recent report suggested that an intracellular granular staining pattern of the extracellular matrix protein nephronectin (NPNT) in breast tumor sections correlated with a poor prognosis. Furthermore, the results showed that NPNT is localized in extracellular vesicles derived from mouse breast cancer cells. In this study, we performed proteomic analysis that revealed that several proteins, including tumor-promoting molecules, are differentially expressed in the cargo of small extracellular vesicles (sEVs) derived from NPNT-expressing mouse breast cancer cells. We also identified three different forms of NPNT at 80, 60, and 20 kDa. We report that the native form of NPNT at 60 kDa becomes further glycosylated and is detected as the 80 kDa NPNT, which may be processed by matrix metalloproteinases to a shorter form of around 20 kDa, which has not previously been described. Although both 80 and 20 kDa NPNT are detected in sEVs derived from breast cancer cells, the 20 kDa form of NPNT is concentrated in sEVs. In summary, we show that a novel truncated form of NPNT is found in sEVs derived from breast cancer cells.
Publisher
American Chemical Society
Citation
Toraskar JP, Norvoll Magnussen S, Hagen L, Sharma A, Hoang L, Bjørkøy GB, Svineng g, Steigedal TS. A Novel Truncated Form of Nephronectin Is Present in Small Extracellular Vesicles Isolated from 66cl4 Cells. Journal of Proteome Research. 2019;18(3):1237-1247
Metadata
Show full item record
Collections
  • Artikler, rapporter og annet (medisinsk biologi) [1105]
Copyright 2019 The Author(s)

Browse

Browse all of MuninCommunities & CollectionsAuthor listTitlesBy Issue DateBrowse this CollectionAuthor listTitlesBy Issue Date
Login

Statistics

View Usage Statistics
UiT

Munin is powered by DSpace

UiT The Arctic University of Norway
The University Library
uit.no/ub - munin@ub.uit.no

Accessibility statement (Norwegian only)