Show simple item record

dc.contributor.authorToraskar, Jimita Prashant
dc.contributor.authorNorvoll Magnussen, Synnøve
dc.contributor.authorHagen, Lars
dc.contributor.authorSharma, Animesh
dc.contributor.authorHoang, Linh
dc.contributor.authorBjørkøy, Geir
dc.contributor.authorSvineng, Gunbjørg
dc.contributor.authorSteigedal, Tonje S.
dc.date.accessioned2020-01-21T09:31:08Z
dc.date.available2020-01-21T09:31:08Z
dc.date.issued2019-02-01
dc.description.abstractExtracellular vesicles are emerging as biomarkers in breast cancer. Our recent report suggested that an intracellular granular staining pattern of the extracellular matrix protein nephronectin (NPNT) in breast tumor sections correlated with a poor prognosis. Furthermore, the results showed that NPNT is localized in extracellular vesicles derived from mouse breast cancer cells. In this study, we performed proteomic analysis that revealed that several proteins, including tumor-promoting molecules, are differentially expressed in the cargo of small extracellular vesicles (sEVs) derived from NPNT-expressing mouse breast cancer cells. We also identified three different forms of NPNT at 80, 60, and 20 kDa. We report that the native form of NPNT at 60 kDa becomes further glycosylated and is detected as the 80 kDa NPNT, which may be processed by matrix metalloproteinases to a shorter form of around 20 kDa, which has not previously been described. Although both 80 and 20 kDa NPNT are detected in sEVs derived from breast cancer cells, the 20 kDa form of NPNT is concentrated in sEVs. In summary, we show that a novel truncated form of NPNT is found in sEVs derived from breast cancer cells.en_US
dc.identifier.citationToraskar JP, Norvoll Magnussen S, Hagen L, Sharma A, Hoang L, Bjørkøy GB, Svineng g, Steigedal TS. A Novel Truncated Form of Nephronectin Is Present in Small Extracellular Vesicles Isolated from 66cl4 Cells. Journal of Proteome Research. 2019;18(3):1237-1247en_US
dc.identifier.cristinIDFRIDAID 1684472
dc.identifier.doi10.1021/acs.jproteome.8b00859
dc.identifier.issn1535-3893
dc.identifier.issn1535-3907
dc.identifier.urihttps://hdl.handle.net/10037/17155
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.journalJournal of Proteome Research
dc.relation.projectIDNotur/NorStore: NS9036Ken_US
dc.relation.urihttps://pubs.acs.org/doi/ipdf/10.1021/acs.jproteome.8b00859
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titleA Novel Truncated Form of Nephronectin Is Present in Small Extracellular Vesicles Isolated from 66cl4 Cellsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record