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Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography

Permanent link
https://hdl.handle.net/10037/17256
DOI
https://doi.org/10.1007/978-1-4939-9133-4_16
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Accepted manuscript version (PDF)
Date
2019
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Hadler-Olsen, Elin Synnøve; Winberg, Jan-Olof
Abstract
To explore the physiological or pathological roles of proteases, it is important to be able to detect and precisely localize them in a tissue, to differentiate between inactive and active forms, as well as to quantify and determine the nature of the enzyme that degrades a given substrate. Here we present a protocol for real-time gelatin zymography that is very useful for the detection of gelatin-degrading proteases in tissue extracts. This method uses fluorescence-labeled gelatin and therefore we also present an easy, fast, and cheap method for labeling gelatin with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF).
Description
This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology, vol 1952. The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-9133-4_16.
Publisher
Humana Press
Series
Methods in Molecular Biology, vol 1952
Citation
Hadler-Olsen E., Winberg JO. (2019) Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography. In: Vigetti D., Theocharis A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952. Humana Press, New York, NY
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  • Artikler, rapporter og annet (medisinsk biologi) [1105]
© Springer Science+Business Media, LLC, part of Springer Nature 2019

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