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dc.contributor.authorHadler-Olsen, Elin Synnøve
dc.contributor.authorWinberg, Jan-Olof
dc.date.accessioned2020-01-29T09:59:35Z
dc.date.available2020-01-29T09:59:35Z
dc.date.issued2019
dc.description.abstractTo explore the physiological or pathological roles of proteases, it is important to be able to detect and precisely localize them in a tissue, to differentiate between inactive and active forms, as well as to quantify and determine the nature of the enzyme that degrades a given substrate. Here we present an in situ gelatin zymography method that allows for a precise localization of active gelatin-degrading enzymes in a tissue section. In this method, dye-quenched gelatin is put on top of a tissue section. During an incubation period, active gelatinolytic enzymes will degrade the substrate and fluorescent signals are emitted from the locations of these enzymes.en_US
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in <i>Methods in Molecular Biology, vol 1952</i>. The final authenticated version is available online at: <a href=https://doi.org/10.1007/978-1-4939-9133-4_15>https://doi.org/10.1007/978-1-4939-9133-4_15. </a>en_US
dc.identifier.citationHadler-Olsen E., Winberg JO. (2019) Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography. In: Vigetti D., Theocharis A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952. Humana Press, New York, NYen_US
dc.identifier.cristinIDFRIDAID 1702654
dc.identifier.doi10.1007/978-1-4939-9133-4_15
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttps://hdl.handle.net/10037/17257
dc.language.isoengen_US
dc.publisherHumana Pressen_US
dc.relation.ispartofseriesMethods in Molecular Biology, vol 1952en_US
dc.relation.journalMethods in molecular biology
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© Springer Science+Business Media, LLC, part of Springer Nature 2019en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleMethod for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymographyen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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