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dc.contributor.authorSmaglyukova, Natalia
dc.contributor.authorSletten, Elise Thoresen
dc.contributor.authorØrbo, Anne
dc.contributor.authorSager, Georg
dc.date.accessioned2021-02-22T21:54:28Z
dc.date.available2021-02-22T21:54:28Z
dc.date.issued2020-06-25
dc.description.abstractA previous investigation showed that the endometrium normalized in women with endometrial hyperplasia after three months treatment with high dose levonorgestrel IUS (intrauterine system). The effect was maintained even if immunohistochemical analyses of the endometrium showed that nuclear progesterone receptors (nPRs) were completely downregulated. These observations indicated that some type of non-genomic effect existed. We conducted new investigations of endometrial hyperplasia, now with 6 months low dose levonorgestrel IUS treatment. Again, the growth disturbances were reversed with normalization of the endometrium. In the context of these studies, RT-qPCR analyses of the endometrium were performed before and after treatment, to determine expression of nuclear progesterone receptors (nPRA+B and nPRB), membrane progesterone receptors (mPR, α-, β- and γ-subtypes) and progesterone receptor membrane components (PGRMC1and PGRMC2). The human cervical cell line (C-4 I) with no detectable nPRs, was included in the investigation as biological control .The gene expression of nPRs, mPRs and PGRMCs was determined in the logarithmic growth phase. Tissue and cellular mRNA was determined with RT-qPCR and used as a surrogate marker for receptor (protein) expression. The present data are connected to the related article entitled <a href=https://hdl.handle.net/10037/20588>“Expression of nuclear progesterone receptors (nPRs), membrane progesterone receptors (mPRs) and progesterone receptor membrane components (PGRMCs) in the human endometrium after 6 months levonorgestrel low dose intrauterine therapy”</a>.en_US
dc.descriptionThe research paper linked to this data paper is available in Munin at <a href=https://hdl.handle.net/10037/20588>https://hdl.handle.net/10037/20588</a>.en_US
dc.identifier.citationSmaglyukova NN, Sletten ET, Ørbo ao, Sager gs. Data on RT-qPCR assay of nuclear progesterone receptors (nPR), membrane progesterone receptors (mPR) and progesterone receptor membrane components (PGRMC) from human uterine endometrial tissue and cancer cells of the uterine cervix. Data in Brief. 2020en_US
dc.identifier.cristinIDFRIDAID 1846690
dc.identifier.doi10.1016/j.dib.2020.105923
dc.identifier.issn2352-3409
dc.identifier.urihttps://hdl.handle.net/10037/20589
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalData in Brief
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Gynecology and obstetrics: 756en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Gynekologi og obstetrikk: 756en_US
dc.titleData on RT-qPCR assay of nuclear progesterone receptors (nPR), membrane progesterone receptors (mPR) and progesterone receptor membrane components (PGRMC) from human uterine endometrial tissue and cancer cells of the uterine cervixen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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