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norskBlar i tittel Institutt for medisinsk biologi
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"I did not intend to stop. I just could not stand cigarettes any more." A qualitative interview study of smoking cessation among the elderly
(Journal article; Tidsskriftartikkel; Peer reviewed, 2011)Every year, more than 650,000 Europeans die because they smoke. Smoking is considered to be the single most preventable factor influencing health. General practitioners (GP) are encouraged to advise on smoking cessation at all suitable consultations. Unsolicited advice from GPs results in one of 40-60 smokers stopping smoking. Smoking cessation advice has traditionally been given on an individual ... -
ICAM1 expression is induced by proinflammatory cytokines and associated with TLS formation in aggressive breast cancer subtypes
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-08-06)Intratumoral formation of tertiary lymphoid structures (TLS) within the tumor microenvironment is considered to be a consequence of antigen challenge during anti-tumor responses. Intracellular adhesion molecule 1 (ICAM1) has been implicated in a variety of immune and inflammatory responses, in addition to associate with triple negative breast cancer (TNBC). In this study, we detected TLS in the ... -
ICESluvan, a 94-Kilobase Mosaic Integrative Conjugative Element Conferring Interspecies Transfer of VanB-Type Glycopeptide Resistance, a Novel Bacitracin Resistance Locus, and a Toxin- Antitoxin Stabilization System
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)A 94-kb integrative conjugative element (ICESluvan) transferable to Enterococcus faecium and Enterococcus faecalis from an animal isolate of Streptococcus lutetiensis consists of a mosaic of genetic fragments from different Gram-positive bacteria. A variant of ICESluvan was confirmed in S. lutetiensis from a patient. A complete Tn5382/Tn1549 with a vanB2 operon is integrated into a streptococcal ... -
Identification and cloning of immunogenic Aliivibrio salmonicida Pal-like protein present in profiled outer membrane and secreted subproteome
(Journal article; Tidsskriftartikkel; Peer reviewed, 2011)Aliivibrio salmonicida is the aetiological agent of cold water vibriosis affecting farmed fish species, a disease that today is fully controlled by vaccination. However, the molecular mechanisms behind the successful vaccine are largely unknown. In order to gain insight into the possible mechanisms of A. salmonicida vaccines, we report here the profiles of both the outer membrane and secreted ... -
Identification and experimental confirmation of novel cGMP efflux inhibitors by virtual ligand screening of vardenafil-analogues
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-28)<i>Background</i> - Clinical studies have reported overexpression of PDE5 and elevation of intracellular cyclic GMP in various types of cancer cells. ABCC5 transports cGMP out of the cells with high affinity. PDE5 inhibitors prevent both cellular metabolism and cGMP efflux by inhibiting ABCC5 as well as PDE5. Increasing intracellular cGMP is hypothesized to promote apoptosis and growth restriction ... -
Identification and follow-up of pregnant women with platelet-type human platelet antigen (HPA)-1bb alloimmunized with fetal HPA-1a
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-11-15)<p><i>Introduction</i>: Pregnant women negative for human platelet antigen 1a (HPA-1a) are at risk of alloimmunization with fetal HPA-1a antigen inherited from the father, and their offspring may develop fetal and neonatal alloimmune thrombocytopenia (FNAIT). The aim of this study was to analyze the frequency of HPA-1a alloimmunization in pregnant Polish women, the feasibility of using maternal ... -
Identification of a core EMT signature that separates basal-like breast cancers into partial- and post-EMT subtypes
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-04)Epithelial-mesenchymal transition (EMT) is a cellular plasticity program critical for embryonic development and tissue regeneration, and aberrant EMT is associated with disease including cancer. The high degree of plasticity in the mammary epithelium is reflected in extensive heterogeneity among breast cancers. Here, we have analyzed RNA-sequencing data from three different mammary epithelial cell ... -
Identification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode large and small T antigens and the 3 structural ... -
Identification of novel serotonin transporter compounds by virtual Screening
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014-02-12)The serotonin (5-hydroxytryptamine, 5-HT) transporter (SERT) plays an essential role in the termination of serotonergic neurotransmission by removing 5-HT from the synaptic cleft into the presynaptic neuron. It is also of pharmacological importance being targeted by antidepressants and psychostimulant drugs. Here, five commercial databases containing approximately 3.24 million drug-like compounds ... -
Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2-JNK signalling in breast cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-01-12)The non-canonical Wnt/planar cell polarity (Wnt/PCP) pathway plays a crucial role in embryonic development. Recent work has linked defects of this pathway to breast cancer aggressiveness and proposed Wnt/PCP signalling as a therapeutic target. Here we show that the archetypal Wnt/PCP protein VANGL2 is overexpressed in basal breast cancers, associated with poor prognosis and implicated in tumour ... -
Identification of protein interaction candidates for the GOLD domain of FYCO1
(Master thesis; Mastergradsoppgave, 2016-05-27)Autophagy is an evolutionary conserved degradative pathway, where damaged or surplus cytosolic components are sequestered into double membrane vesicles, autophagosomes, which become degraded through the lysosomal system. The autophagy is a dynamic process, which is depended of transport of autophagosomes along microtubule, to become degraded by lysosomes. One of the proteins involved in this transport ... -
Identification of VIM-2-producing Pseudomonas aeruginosa from Tanzania is associated with sequence types 244 and 640 and the location of blaVIM-2 in a TniC integron
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Identification, isolation and characterisation of scavenging endothelial cells in placenta
(Master thesis; Mastergradsoppgave, 2014-05-15)Haemopoiesis occurs in the BM, where haemopoietic stem cells (HSC) reside in a three-dimensional “niche” and give rise to the millions of circulating blood cells required everyday of life. The precise regulation of this immense task occurs by interaction of HSC with other BM cells and extracellular matrix molecules. In addition, the BM vasculature has also now been identified as a key HSC regulator. ... -
Identifying Staphylococcus haemolyticus surface proteins. Development of a novel method for detection of bacterial surface proteins expressed during colonisation of human keratinocytes
(Master thesis; Mastergradsoppgave, 2018-05-15)The aim was to develop a method for identification of expressed surface proteins of Staphylococcus haemolyticus when colonising human keratinocytes. S. haemolyticus and HaCaT cells were grown together and separated with FACS prior to surface protein shaving using the LPI™FlowCell technology (Nanoxis Consulting AB). TMT-tags were used to investigate up- and downregulation of proteins comparing S. ... -
IL-1β Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02-06)Recently we described that endonuclease inactive DNase I translocated into the nucleus in response to increased endogenous IL-1β expression. Here, we demonstrate impact and function of translocated DNase I in tubular cells. Effect of cytokines on expression level and nuclear localisation of DNase I and corresponding levels of Fas receptor (FasR) and IL-1β were determined by confocal microscopy, qPCR ... -
Immune-modulating properties of carcinoma-associated fibroblasts and their changes during radiotherapy. In vitro study of primary non-small cell lung carcinoma-associated fibroblasts
(Master thesis; Mastergradsoppgave, 2015-05-15)Background/aim of the study: Carcinoma-associated Fibroblasts (CAFs) comprise a heterogeneous population of cells and often represent the major cellular component of solid malignancies playing critical roles in tumor progression and metastasis. The purpose of this study was to gain more knowledge on the immunosuppressive traits of CAFs isolated from non-small cell lung carcinomas (NSCLC), and to ... -
Immunization with lytic polysaccharide monooxygenase CbpD induces protective immunity against Pseudomonas aeruginosa pneumonia
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-17)Pseudomonas aeruginosa (PA) CbpD belongs to the lytic polysaccharide monooxygenases (LPMOs), a family of enzymes that cleave chitin or related polysaccharides. Here, we demonstrate a virulence role of CbpD in PA pneumonia linked to impairment of host complement function and opsonophagocytic clearance. Following intratracheal challenge, a PA ΔCbpD mutant was more easily cleared and produced less ... -
The immunophilin Zonda controls regulated exocytosis in endocrine and exocrine tissues
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-18)Exocytosis is a fundamental process in physiology, that ensures communication between cells, organs and even organisms. Hormones, neuropeptides and antibodies, among other cargoes are packed in exocytic vesicles that need to reach and fuse with the plasma membrane to release their content to the extracellular milieu. Hundreds of proteins participate in this process and several others in its regulation. ... -
The Immunostimulatory Potential of Mesenchymal Stem Cells for formation of Tertiary Lymphoid Structures in Lupus Nephritis
(Master thesis; Mastergradsoppgave, 2017-05-15)Formation of tertiary lymphoid structures (TLS) occurs in tissues targeted by chronic inflammatory processes, such as infection and autoimmunity. In systemic lupus erythematosus (SLE), TLS have been observed in the kidneys of lupus-prone mice and in kidney biopsies of SLE patients with Lupus Nephritis (LN). Here the role of tissue-specific mesenchymal stem cells (MSCs) as lymphoid tissue organizer ... -
Impact of KRAS, BRAF and microsatellite instability status after cytoreductive surgery and HIPEC in a national cohort of colorectal peritoneal metastasis patients
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-09)Background - Patients with metastatic colorectal cancer (mCRC) carrying BRAF (mutBRAF) or KRAS mutation (mutKRAS) have an inferior prognosis after liver or lung surgery, whereas the prognostic role in the context of peritoneal metastasis (PM) after cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) has been less investigated.<p> <p>Methods - In total, 257 patients ...
