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dc.contributor.authorCavanagh, Jorunn Pauline
dc.contributor.authorKlingenberg, Claus Andreas
dc.contributor.authorHanssen, Anne-Merethe
dc.contributor.authorAarag, Elizabeth
dc.contributor.authorFrancois, Patrice
dc.contributor.authorSchrenzel, Jaques
dc.contributor.authorFlægstad, Trond
dc.contributor.authorSollid, Johanna U Ericson
dc.date.accessioned2012-07-13T08:29:56Z
dc.date.available2012-07-13T08:29:56Z
dc.date.issued2012
dc.description.abstractThe notoriously multi-resistant Staphylococcus haemolyticus is an emerging pathogen causing serious infections in immunocompromised patients. Defining the population structure is important to detect outbreaks and spread of antimicrobial resistant clones. Currently, the standard typing technique is pulsed-field gel electrophoresis (PFGE). In this study we describe novel molecular typing schemes for S. haemolyticus using multi locus sequence typing (MLST) and multi locus variable number of tandem repeats (VNTR) analysis. Seven housekeeping genes (MLST) and five VNTR loci (MLVF) were selected for the novel typing schemes. A panel of 45 human and veterinary S. haemolyticus isolates was investigated. The collection had diverse PFGE patterns (38 PFGE types) and was sampled over a 20 year-period from eight countries. MLST resolved 17 sequence types (Simpsons index of diversity [SID] = 0.877) and MLVF resolved 14 repeat types (SID = 0.831). We found a low sequence diversity. Phylogenetic analysis clustered the isolates in three (MLST) and one (MLVF) clonal complexes, respectively. Taken together, neither the MLST nor the MLVF scheme was suitable to resolve the population structure of this S. haemolyticus collection. Future MLVF and MLST schemes will benefit from addition of more variable core genome sequences identified by comparing different fully sequenced S. haemolyticus genomes.en
dc.identifier.citationJournal of Microbiological Methods 89(2012) nr. 3 s. 159-166en
dc.identifier.cristinIDFRIDAID 933993
dc.identifier.doidoi: 10.1016/j.mimet.2012.03.014
dc.identifier.issn0167-7012
dc.identifier.urihttps://hdl.handle.net/10037/4354
dc.identifier.urnURN:NBN:no-uit_munin_4072
dc.language.isoengen
dc.publisherElsevier Scienceen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical molecular biology: 711en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715en
dc.titleCore genome conservation of Staphylococcus haemolyticus limits sequence based population structure analysisen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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