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dc.contributor.authorUrbarova, Ilona
dc.contributor.authorKarlsen, Bård Ove
dc.contributor.authorOkkenhaug, Siri
dc.contributor.authorSeternes, Ole Morten
dc.contributor.authorJohansen, Steinar
dc.contributor.authorEmblem, Åse
dc.date.accessioned2013-03-08T07:13:46Z
dc.date.available2013-03-08T07:13:46Z
dc.date.issued2012-10-18
dc.description.abstractMarine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia digitata. We generated approximately 1.1 million quality-filtered sequencing reads by 454 pyrosequencing, which were assembled into approximately 120,000 contigs and 220,000 single reads. Based on annotation and gene ontology analysis we profiled the expressed mRNA transcripts according to known biological processes. As a proof-of-concept we identified polypeptide toxins with a potential blocking activity on sodium and potassium voltage-gated channels from digital transcriptome libraries.
dc.identifier.citationMarine Drugs 10(2012) nr. 10 s. 2265-2279en
dc.identifier.cristinIDFRIDAID 974693
dc.identifier.doihttp://dx.doi.org/10.3390/md10102265
dc.identifier.issn1660-3397
dc.identifier.urihttps://hdl.handle.net/10037/4917
dc.identifier.urnURN:NBN:no-uit_munin_4637
dc.language.isoengen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en
dc.subjectVDP::Mathematics and natural science: 400::Chemistry: 440::Environmental chemistry, natural environmental chemistry: 446en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Kjemi: 440::Miljøkjemi, naturmiljøkjemi: 446en
dc.titleDigital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemonesen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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