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dc.contributor.authorWilzén, Annika
dc.contributor.authorKrona, Cecilie
dc.contributor.authorSveinbjørnsson, Baldur
dc.contributor.authorKristiansson, Erik
dc.contributor.authorDalevi, Daniel
dc.contributor.authorØra, Ingrid
dc.contributor.authorDe Preter, Katleen
dc.contributor.authorStallings, Raymond L.
dc.contributor.authorMaris, John
dc.contributor.authorVersteeg, Rogier
dc.contributor.authorNilsson, Staffan
dc.contributor.authorKogner, Per
dc.contributor.authorAbel, Frida
dc.date.accessioned2014-02-27T07:41:05Z
dc.date.available2014-02-27T07:41:05Z
dc.date.issued2013
dc.description.abstractBackground: Neuroblastoma (NB) tumours are commonly divided into three cytogenetic subgroups. However, by unsupervised principal components analysis of gene expression profiles we recently identified four distinct subgroups, r1-r4. In the current study we characterized these different subgroups in more detail, with a specific focus on the fourth divergent tumour subgroup (r4). Methods: Expression microarray data from four international studies corresponding to 148 neuroblastic tumour cases were subject to division into four expression subgroups using a previously described 6-gene signature. Differentially expressed genes between groups were identified using Significance Analysis of Microarray (SAM). Next, gene expression network modelling was performed to map signalling pathways and cellular processes representing each subgroup. Findings were validated at the protein level by immunohistochemistry and immunoblot analyses. Results: We identified several significantly up-regulated genes in the r4 subgroup of which the tyrosine kinase receptor ERBB3 was most prominent (fold change: 132–240). By gene set enrichment analysis (GSEA) the constructed gene network of ERBB3 (n = 38 network partners) was significantly enriched in the r4 subgroup in all four independent data sets. ERBB3 was also positively correlated to the ErbB family members EGFR and ERBB2 in all data sets, and a concurrent overexpression was seen in the r4 subgroup. Further studies of histopathology categories using a fifth data set of 110 neuroblastic tumours, showed a striking similarity between the expression profile of r4 to ganglioneuroblastoma (GNB) and ganglioneuroma (GN) tumours. In contrast, the NB histopathological subtype was dominated by mitotic regulating genes, characterizing unfavourable NB subgroups in particular. The high ErbB3 expression in GN tumour types was verified at the protein level, and showed mainly expression in the mature ganglion cells. Conclusions: Conclusively, this study demonstrates the importance of performing unsupervised clustering and subtype discovery of data sets prior to analyses to avoid a mixture of tumour subtypes, which may otherwise give distorted results and lead to incorrect conclusions. The current study identifies ERBB3 as a clear-cut marker of a GNB/GN-like expression profile, and we suggest a 7-gene expression signature (including ERBB3) as a complement to histopathology analysis of neuroblastic tumours. Further studies of ErbB3 and other ErbB family members and their role in neuroblastic differentiation and pathogenesis are warranted.en
dc.identifier.citationMolecular Cancer (2013), vol. 12:70en
dc.identifier.cristinIDFRIDAID 1072674
dc.identifier.doihttp://dx.doi.org/10.1186/1476-4598-12-70
dc.identifier.issn1476-4598
dc.identifier.urihttps://hdl.handle.net/10037/5878
dc.identifier.urnURN:NBN:no-uit_munin_5573
dc.language.isoengen
dc.publisherBioMed Centralen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en
dc.titleERBB3 is a marker of a ganglioneuroblastoma/ganglioneuroma-like expression profile in neuroblastic tumoursen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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