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dc.contributor.authorSimonato, Francesca
dc.contributor.authorVentura, Laura
dc.contributor.authorSartori, Nicola
dc.contributor.authorCappellesso, Rocco
dc.contributor.authorFassan, Matteo
dc.contributor.authorBusund, Lill-Tove
dc.contributor.authorFassina, Ambrogio
dc.date.accessioned2014-03-21T07:20:04Z
dc.date.available2014-03-21T07:20:04Z
dc.date.issued2013
dc.description.abstractMicroRNAs’ dysregulation and profiling have been demonstrated to be clinically relevant in urothelial carcinoma (UC). Urine cytology is commonly used as the mainstay non-invasive test for secondary prevention and follow-up of UC patients. Ancillary tools are needed to support cytopathologists in the diagnosis of low-grade UC. The feasibility and reliability of microRNAs profiling by qRT-PCR analysis (miR-145 and miR-205) in archival routine urine cytology smears (affected by fixation/staining [Papanicolau] and room temperature storage) was tested in a series of 15 non-neoplastic and 10 UC urine specimens. Only samples with > 5,000 urothelial cells and with ,50% of inflammatory cells/red blood cells clusters were considered. Overall, a satisfactory amount of total RNA was obtained from all the considered samples (mean 1.2761.43 mg, range 0.06–4.60 mg). Twenty nanograms of total RNA have been calculated to be the minimal total RNA concentration for reliable and reproducible miRNAs expression profiling analysis of archival cytological smears (slope = -3.4084; Rsquared = 0.99; efficiency = 1.94). miR-145 and miR-205 were significantly downregulated in UC samples in comparison to non-tumor controls. These findings demonstrate that urine archival cytology smears are suitable for obtaining high-quality RNA to be used in microRNAs expression profiling. Further studies should investigate if miRNAs profiling can be successfully translated into clinical practice as diagnostic or prognostic markers.en
dc.identifier.citationPLoS ONE (2013), vol. 8(2): e57490en
dc.identifier.cristinIDFRIDAID 1031489
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0057490
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/6030
dc.identifier.urnURN:NBN:no-uit_munin_5751
dc.language.isoengen
dc.publisherPublic Library of Science (PLoS)en
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical genetics: 714en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714en
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::General pathology, anatomical pathology: 719en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Generell patologi, patologisk anatomi: 719en
dc.titleDetection of MicroRNAs in Archival Cytology Urine Smearsen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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