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dc.contributor.authorHadler-Olsen, Elin Synnøve
dc.contributor.authorSolli, Ann Iren
dc.contributor.authorHafstad, Anne Dragøy
dc.contributor.authorWinberg, Jan-Olof
dc.contributor.authorUhlin-Hansen, Lars
dc.date.accessioned2015-08-26T12:20:19Z
dc.date.available2015-09-29T05:50:04Z
dc.date.issued2014-09-29
dc.description.abstractMatrix metalloproteinase 2 (MMP-2) is a proteolytic enzyme implicated in motility, differentiation, and regeneration of skeletal muscle fibers through processing of extracellular substrates. Although MMP-2 has been found to be localized intracellularly in cardiomyocytes where the enzyme is thought to contribute to post-ischemic loss of contractility, little is known about intracellular MMP-2 activity in skeletal muscle fibers. In the present study we demonstrate intracellular MMP-2 in normal skeletal muscle by immunohistochemical staining. Immunogold electron microscopic analyses indicated that the enzyme was concentrated in Z-lines of the sarcomers, in the nuclear membrane, and in mitochondria. By use of in situ zymography, we found that gelatinolytic activity in muscle fibers was co-localized with immunofluorecent staining for MMP-2. Staining for MMP-9, the other member of the gelatinase group of the MMPs, was negative. The broad-spectrum metalloprotease inhibitor EDTA and the selective gelatinase inhibitor CTT2, but not the cysteine inhibitor E64, strongly reduced the gelatinolytic activity. The intracellular gelatinolytic activity was much more prominent in fast twitch type II fibers than in slow twitch type I fibers, and there was a decrease in intracellular gelatinolytic activity and MMP-2 expression in muscles from mice exposed to high intensity interval training. Together our results indicate that MMP-2 is part of the intracellular proteolytic network in normal skeletal muscle, especially in fast twitch type II fibers. Further, the results suggest that intracellular MMP-2 in skeletal muscle fibers is active during normal homeostasis, and affected by the level of physical activity.en_US
dc.identifier.citationJournal of Cellular Physiology, Volume 230, Issue 1, pages 160–169, January 2015en_US
dc.identifier.cristinIDFRIDAID 1152473
dc.identifier.doi10.1002/jcp.24694
dc.identifier.issn0021-9541
dc.identifier.urihttps://hdl.handle.net/10037/7980
dc.identifier.urnURN:NBN:no-uit_munin_7560
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rights.accessRightsopenAccess
dc.subjectxxen_US
dc.titleIntracellular MMP-2 Activity in Skeletal Muscle is Associated with Type II Fibersen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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