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dc.contributor.authorNygård, Ingvild
dc.contributor.authorMortensen, Kim Erlend
dc.contributor.authorHedegaard, Jakob
dc.contributor.authorConley, Lene Nagstrup
dc.contributor.authorBendixen, Christian
dc.contributor.authorSveinbjørnsson, Baldur
dc.contributor.authorRevhaug, Arthur
dc.date.accessioned2016-02-18T12:56:39Z
dc.date.available2016-02-18T12:56:39Z
dc.date.issued2015-06
dc.description.abstractAim. To study genes regulating the extracellular matrix (ECM) and investigate the tissue remodelling following liver resection in porcine. <p>Methods. Four pigs with 60% partial hepatectomy- (PHx-) induced liver regeneration were studied over six weeks. Four pigs underwent sham surgery and another four pigs were used as controls of the normal liver growth. Liver biopsies were taken upon laparotomy, after three and six weeks. Gene expression profiles were obtained using porcine-specific oligonucleotide microarrays. Immunohistochemical staining was performed and a proliferative index was assessed. <p>Results. More differentially expressed genes were associated with the regulation of ECM in the resection group compared to the sham and control groups. Secreted protein acidic and rich in cysteine (SPARC) and collagen 1, alpha 2 (COL1A2) were both upregulated in the early phase of liver regeneration, validated by immunopositive cells during the remodelling phase of liver regeneration. A broadened connective tissue was demonstrated by Masson’s Trichrome staining, and an immunohistochemical staining against pan-Cytokeratin (panCK) demonstrated a distinct pattern of migrating cells, followed by proliferating cell nuclear antigen (PCNA) positive nuclei. <p>Conclusions. The present study demonstrates both a distinct pattern of PCNA positive nuclei and a deposition of ECM proteins in the remodelling phase of liver regeneration.en_US
dc.identifier.citationBioMed Research International 2015, 2015:248920en_US
dc.identifier.cristinIDFRIDAID 1278152
dc.identifier.doi10.1155/2015/248920
dc.identifier.issn2314-6141
dc.identifier.urihttps://hdl.handle.net/10037/8509
dc.identifier.urnURN:NBN:no-uit_munin_8079
dc.language.isoengen_US
dc.publisherHindawi Publishing Corporationen_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750en_US
dc.titleTissue remodelling following resection of porcine liveren_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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