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dc.contributor.authorCiuffa, Rodolfo
dc.contributor.authorLamark, Trond
dc.contributor.authorTarafder, Abul K.
dc.contributor.authorGuesdon, Audrey
dc.contributor.authorRybina, Sofia
dc.contributor.authorHagen, Wim J.H.
dc.contributor.authorJohansen, Terje
dc.contributor.authorSachse, Carsten
dc.date.accessioned2016-03-01T12:31:37Z
dc.date.available2016-03-01T12:31:37Z
dc.date.issued2015-05-05
dc.description.abstractThe scaffold protein p62/SQSTM1 is involved in protein turnover and signaling and is commonly found in dense protein bodies in eukaryotic cells. In autophagy, p62 acts as a selective autophagy receptor that recognizes and shuttles ubiquitinated proteins to the autophagosome for degradation. The structural organization of p62 in cellular bodies and the interplay of these assemblies with ubiquitin and the autophagic marker LC3 remain to be elucidated. Here, we present a cryo-EM structural analysis of p62. Together with structures of assemblies from the PB1 domain, we show that p62 is organized in flexible polymers with the PB1 domain constituting a helical scaffold. Filamentous p62 is capable of binding LC3 and addition of long ubiquitin chains induces disassembly and shortening of filaments. These studies explain how p62 assemblies provide a large molecular scaffold for the nascent autophagosome and reveal how they can bind ubiquitinated cargo.en_US
dc.descriptionPublished version also available at <a href=http://dx.doi.org/10.1016/j.celrep.2015.03.062>http://dx.doi.org/10.1016/j.celrep.2015.03.062</a>en_US
dc.identifier.citationCell reports 2015, 11(5):748-758en_US
dc.identifier.cristinIDFRIDAID 1259942
dc.identifier.doi10.1016/j.celrep.2015.03.062
dc.identifier.issn2211-1247
dc.identifier.urihttps://hdl.handle.net/10037/8591
dc.identifier.urnURN:NBN:no-uit_munin_8201
dc.language.isoengen_US
dc.publisherElsevier (Cell Press)en_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical molecular biology: 711en_US
dc.titleThe Selective Autophagy Receptor p62 Forms a Flexible Filamentous Helical Scaffolden_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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