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dc.contributor.authorEl-Gewely, Mohamed Raafat
dc.contributor.authorAndreassen, Morten
dc.contributor.authorWalquist, Mari
dc.contributor.authorUrsvik, Anita
dc.contributor.authorKnutsen, Erik
dc.contributor.authorNystad, Mona
dc.contributor.authorCoucheron, Dag H
dc.contributor.authorMyrmel, Kristin Smistad
dc.contributor.authorHennig, Rune
dc.contributor.authorJohansen, Steinar D
dc.date.accessioned2016-08-24T09:08:06Z
dc.date.available2016-08-24T09:08:06Z
dc.date.issued2016-03-03
dc.description.abstractMeningiomas represent the most common primary tumors of the central nervous system, but few microRNA (miRNA) profiling studies have been reported so far. Deep sequencing of small RNA libraries generated from two human meningioma biopsies WHO grades I (benign) and II (atypical) were compared to excess dura controls. Nineteen differentially expressed miRNAs were validated by RT-qPCR using tumor RNA from 15 patients and 5 meninges controls. Tumor suppressor miR-218 and miR-34a were upregulated relative to normal controls, however, miR-143, miR-193b, miR-451 and oncogenic miR-21 were all downregulated. From 10 selected putative mRNA targets tested by RT-qPCR only four were differentially expressed relative to normal controls. PTEN and E-cadherin (CDH1) were upregulated, but RUNX1T1 was downregulated. Proliferation biomarker p63 was upregulated with nuclear localization, but not detected in most normal arachnoid tissues. Immunoreactivity of E-cadherin was detected in the outermost layer of normal arachnoids, but was expressed throughout the tumors. Nuclear Cyclin D1 expression was positive in all studied meningiomas, while its expression in arachnoid was limited to a few trabecular cells. Meningiomas of grades I and II appear to share biomarkers with malignant tumors, but with some additional tumor suppressor biomarkers expression. Validation in more patients is of importance.en_US
dc.descriptionPublisher's version, source <a href=http://doi.org/10.3390/cancers8030031>http://doi.org/10.3390/cancers8030031</a>en_US
dc.identifier.citationCancers 2016, 8(3), 31en_US
dc.identifier.cristinIDFRIDAID 1340031
dc.identifier.doi10.3390/cancers8030031
dc.identifier.issn2072-6694
dc.identifier.urihttps://hdl.handle.net/10037/9525
dc.identifier.urnURN:NBN:no-uit_munin_9082
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en_US
dc.subjectmeningiomaen_US
dc.subjectSOLiD deep sequencingen_US
dc.subjectmiRNAen_US
dc.subjectRT-qPCRen_US
dc.subjectcap cells and Immunohistochemistry (IHC)en_US
dc.titleDifferentially Expressed MicroRNAs in Meningiomas Grades I and II Suggest Shared Biomarkers with Malignant Tumorsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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