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dc.contributor.authorZischka, Melanie
dc.contributor.authorKünne, Carsten T.
dc.contributor.authorBlom, Jochen
dc.contributor.authorWobser, Dominique
dc.contributor.authorSakιnç, Türkân
dc.contributor.authorSchmidt-Hohagen, Kerstin
dc.contributor.authorDabrowski, P. Wojtek
dc.contributor.authorNitsche, Andreas
dc.contributor.authorHübner, Johannes
dc.contributor.authorHain, Torsten
dc.contributor.authorChakraborty, Trinad
dc.contributor.authorLinke, Burkhard
dc.contributor.authorGoesmann, Alexander
dc.contributor.authorVoget, Sonja
dc.contributor.authorDaniel, Rolf
dc.contributor.authorSchomburg, Dietmar
dc.contributor.authorHauck, Rüdiger
dc.contributor.authorHafez, Hafez M.
dc.contributor.authorTielen, Petra
dc.contributor.authorJahn, Dieter
dc.contributor.authorSolheim, Margrete
dc.contributor.authorSadowy, Ewa
dc.contributor.authorLarsen, Jesper
dc.contributor.authorJensen, Lars Bogø
dc.contributor.authorRuiz-Garbajosa, Patricia
dc.contributor.authorPérez, Dianelys Quiñones
dc.contributor.authorMikalsen, Theresa
dc.contributor.authorBender, Jennifer
dc.contributor.authorSteglich, Matthias
dc.contributor.authorNübel, Ulrich
dc.contributor.authorWitte, Wolfgang
dc.contributor.authorWerner, Guido
dc.date.accessioned2015-08-26T11:50:19Z
dc.date.available2015-08-26T11:50:19Z
dc.date.issued2015-03-12
dc.description.abstractBackground: Enterococcus faecalis is a multifaceted microorganism known to act as a beneficial intestinal commensal bacterium. It is also a dreaded nosocomial pathogen causing life-threatening infections in hospitalised patients. Isolates of a distinct MLST type ST40 represent the most frequent strain type of this species, distributed worldwide and originating from various sources (animal, human, environmental) and different conditions (colonisation/infection). Since enterococci are known to be highly recombinogenic we determined to analyse the microevolution and niche adaptation of this highly distributed clonal type. <br>Results: We compared a set of 42 ST40 isolates by assessing key molecular determinants, performing whole genome sequencing (WGS) and a number of phenotypic assays including resistance profiling, formation of biofilm and utilisation of carbon sources. We generated the first circular closed reference genome of an E. faecalis isolate D32 of animal origin and compared it with the genomes of other reference strains. D32 was used as a template for detailed WGS comparisons of high-quality draft genomes of 14 ST40 isolates. Genomic and phylogenetic analyses suggest a high level of similarity regarding the core genome, also demonstrated by similar carbon utilisation patterns. Distribution of known and putative virulence-associated genes did not differentiate between ST40 strains from a commensal and clinical background or an animal or human source. Further analyses of mobile genetic elements (MGE) revealed genomic diversity owed to: (1) a modularly structured pathogenicity island; (2) a site-specifically integrated and previously unknown genomic island of 138 kb in two strains putatively involved in exopolysaccharide synthesis; and (3) isolate-specific plasmid and phage patterns. Moreover, we used different cell-biological and animal experiments to compare the isolate D32 with a closely related ST40 endocarditis isolate whose draft genome sequence was also generated. D32 generally showed a greater capacity of adherence to human cell lines and an increased pathogenic potential in various animal models in combination with an even faster growth in vivo (not in vitro). <br>Conclusion: Molecular, genomic and phenotypic analysis of representative isolates of a major clone of E. faecalis MLST ST40 revealed new insights into the microbiology of a commensal bacterium which can turn into a conditional pathogen.en_US
dc.identifier.citationBMC Genomics 2015, 16:175en_US
dc.identifier.cristinIDFRIDAID 1253222
dc.identifier.doiDOI: 10.1186/s12864-015-1367-x
dc.identifier.issn1471-2164
dc.identifier.urihttps://hdl.handle.net/10037/7976
dc.identifier.urnURN:NBN:no-uit_munin_7562
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.rights.accessRightsopenAccess
dc.subjectEnterococcus faecalisen_US
dc.subjectWhole genomeen_US
dc.subjectEspen_US
dc.subjectPathogenicity islanden_US
dc.subjectCapsuleen_US
dc.titleComprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40en_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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